14-12_peptide top banner
 
Molecular Biology Reagents  >  Water and Molecular Biology Solutions
 

ELISA Microwell Blocking Buffer with Stabilizer

NCI Collaboration
Order Offline
Rockland Immunochemicals produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabalizer (p/n MB-063-0100) added to the microwell plate and incubated overnight at 4°C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n MB-064-1000) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n MB-066-0100), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer (p/n MB-060-0100), added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB ELISA Peroxidase Substrate (p/n TMBE-1000) was added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm.
View All Images
Number:
MB-064-0100
Size:
100 mL
Price:
$65.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

ELISA Microwell Blocking Buffer with Stabilizer Properties

Description
ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free) - MB-064-0100
Application
ELISA : Yes
Other Dilution: User Optimized
Product Type
Buffer
Product Type
Solution
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Label
Buffer
See application note.
Download CofA:
Lot#  
Print

ELISA Microwell Blocking Buffer with Stabilizer Description

Background
ELISA Microwell Blocking Buffer with Stabilizer is designed to block microwells coated with antigens, antibodies or other ligands and stablize the plates for drying.  ELISA stabilizer allows the user to dry and store the plates for a minimum of one (1) year without significant loss of signal.
Storage Condition
Store container at 4° C before opening. Protect from moisture and light. No special shipping conditions or precautions are required.
Application Note
This product is a ''ready-to-use'' 1X solution for stabilizing ELISA plates for storage prior to ELISA. After coating with antigen or antibody, wash the contents of the ELISA microwells and add a sufficient volume of ELISA Microwell Blocking Buffer with Stabilizer to each well of the microplate. Let stand for 2h at room temperature. Aspirate contents of each well and allow the plate to dry. Seal the plate and store appropriately for future use. This buffer contains phosphate buffered saline, and proprietary reagents to block and stabilize ELISA plates. A proprietary combination of stabilizers and preservatives are used that is azide and mercury free.
Purity/Specifity
This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry