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2X SDS-PAGE Sample Buffer without DTT or b-MeOH

NCI Collaboration
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Number:
MB-018
Size:
100 mL
Price:
$65.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

2X SDS-PAGE Sample Buffer without DTT or b-MeOH Properties

Description
2X SDS-PAGE Sample Buffer without DTT or b-MeOH (125 mM Tris HCl, 20% Glycerol, 4% SDS, 0.1% bromphenol blue, pH 6.8) - MB-018
Application
Other Dilution: User Optimized
Product Type
Buffer
Product Type
Solution
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Label
Concentration
2X N/A
Buffer
See application note.
Preservative
None
Stabilizer
None
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2X SDS-PAGE Sample Buffer without DTT or b-MeOH Description

Synonyms
Sample buffer for protein electrophoresis.
Storage Condition
Store container at -20° C or colder prior to opening. Aliquot and re-freeze for best results. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.
Application Note
This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 2X SDS-PAGE Sample Buffer consists of 0.125 M Tris-HCl, 4% (w/v) SDS, 20% (v/v) Glycerol and 0.01% (w/v) bromphenol blue.  The pH of this solution is 6.8.  Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
Purity/Specifity
This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry