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Kits  >  Colorimetric Western Blot Kits
 

Colorimetric WBlot Kit: TMB/DAB Substrate Kit for WBlot using Human Primary Ab MaxTag™

Code

KIA001
Size

1 Kit
Price

$245.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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TMB/DAB Colorimetric Western Blotting Kits can generate publication quality results for novices and experts alike.  Expect reliable and reproducible signal with low back-ground staining.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Western Blotting Kit, TMB Kit, DAB Kit, Peroxidase Kit, Immunoblotting Kit

Colorimetric WBlot Kit: TMB/DAB Substrate Kit for WBlot using Human Primary Ab MaxTag™ Properties

Description
Colorimetric Western Blot Kit: TMB/DAB Substrate Kit for Western Blot using Human Primary Antibody MaxTag™ - KIA001
Known Cross Reactivity
Anti-Human IgG
Application
Western Blot : 1:2,000
Other Dilution: User Optimized
Product Type
Kit
Kit Type
Colorimetric Western Blot Kit
Physical State
n/a
Shipping condition
Ambient
Label
Preservative
Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
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Lot#  

Colorimetric WBlot Kit: TMB/DAB Substrate Kit for WBlot using Human Primary Ab MaxTag™ Description

Background
This western blotting kit allows for the detection of primary human polyclonal or monoclonal antibody provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. The first step in the detection of the membrane bound primary antibody-antigen complex is the addition of a secondary antibody that has been affinity purified, cross-adsorbed, and biotinylated. This biotinylated secondary antibody is visualized after reaction with streptavidin-peroxidase conjugate and subsequent addition of either TMB or DAB substrates. The increased sensitivity and low background achieved by Rockland Immunochemicals' MaxTag? Western Blotting Kit relies on the use of an enhanced streptavidin-peroxidase conjugate and a highly active biotinylated antibody. The streptavidin carries a neutral charge and fewer carbohydrate groups than positively charged avidin. The biotin incorporates a ''spacer'' group that allows for better accessibility of the streptavidin-peroxidase conjugate.
Storage Condition
See kit insert for complete instructions.
Application Note
MaxTag western blotting kit provides a rapid, sensitive, and economical way to develop western blots. The design of this kit simplifies the process of western blotting applications. Straightforward procedures and color-coding add to the ease of use. Two substrates are provided for flexibility, TMB substrate producing a bright blue color and DAB substrate producing a dark brown color. This kit can process up to 40 blots (100 cm2) and is stable for at least 1 year when stored at +4°C.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
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Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry