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Kits  >  Chemiluminescent Western Blot Kits
 

HRP Western Blot Anti-Mouse IgG Antibody

Goat Polyclonal
Code

KCB002
Size

100 µg
Price

$65.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Shipping:
$20.00 to United States
Handling:
$20.00
Synonyms: Anti-mouse IgG HRP conjugated, HRP-linked Antibody, Anti-Mouse HRP Secondary Antibody, Horseradish Peroxidase-Conjugated Antibody

HRP Western Blot Anti-Mouse IgG Antibody Properties

Description
HRP Western Blot Anti-Mouse IgG Antibody - KCB002
Host
Goat
Known Cross Reactivity
n/a
Clonality
Polyclonal
Application
Western Blot : Western Blot Analysis
Product Type
Component
Kit Type
Chemiluminescent Western Blot Kit
Shipping condition
Wet Ice
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
GeneName
n/a
Download CofA
Lot#  

HRP Western Blot Anti-Mouse IgG Antibody Description

Background
HRP is used in applications primarily for its ability to amplify a weak signal and increase detectability of a target molecule. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal and HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times. Chemiluminescence is the best all-around method for western blot detection as these systems eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. These methods are also unmatched in the speed and sensitivity compared to traditional alternatives, utilizing film to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. This item is a component of kit (KCA002). The Kit contains all the reagents necessary to perform chemiluminescent Western Blots to detect mouse primary antibodies.
Storage Condition
See kit insert for complete instructions.
Application Note
Western Blot Analysis
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate