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GFP and Epitopes  >  Antibodies to More Epitope Tags
 

Epitope Tag Antibody Sampler Kit

Code

K915
Size

1 Kit
Price

$425.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Rockland produces kits to meet the requirements of researchers for highly sensitive chemiluminescence and other antibody based assays.
Anti-6X His epitope tag polyclonal antibody (600-401-382) detects His-tagged recombinant proteins by western blot.  The blot was blocked with 3% BSA in TBST for 45 min at RT.  Antibody was incubated with blot at a 1:1,000 dilution in TBST with 3% BSA for 1 hour at RT.  Detection occurred using HRP Gt-a-Rabbit IgG (p/n 611-103-127) diluted 1:80,000 in blocking buffer (p/n MB-070) for 30 min at RT.  Lane 1 was loaded with 12-Epitope Tag Protein Marker Lysate (p/n MB-301-0100) which has the His epitope tag incorporated through a C-terminal linkage (~42 kDa).  Lane 2 was loaded with His-SUMO-GFP recombinant protein which has the His epitope tag incorporated through an N-terminal linkage (~40 kDa).  A 4-20% gradient gel was used to resolve the protein by SDS-PAGE.  Proteins were transferred to nitrocellulose using standard methods.  Molecular weights were estimated by comparison to standards (lane M).
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Synonyms: FLAG, Green Fluorescent Protein, GFP, GST, six histidine, 6xHis

Epitope Tag Antibody Sampler Kit Properties

Description
EPITOPE TAG ANTIBODY SAMPLER KIT - K915
Known Cross Reactivity
6X HIS, FLAG, GFP, GST tagged proteins
Application
ELISA : User Optimized
ChIP : User Optimized
IF Microscopy : User Optimized
Flow Cytometry : User Optimized
Western Blot : User Optimized
Immunohistochemistry: User Optimized
ImmunoPrecipitation: User Optimized
Shipping condition
Dry Ice
Label
Download CofA
Lot#  

Epitope Tag Antibody Sampler Kit Description

Background
Epitope Tag Antibody Sampler Kit detects overexpressed fusion proteins containing the corresponding epitope tags. DYKDDDDK Tag Antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies). Epitope tags are useful for the labeling and detection of proteins using western blotting, immunoprecipitation, and immunostaining techniques
Immunogen
GFP, GST, DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies), 6X His peptide
Immunogen Type
Peptide
Storage Condition
Store Epitope Tag Kit at -20° C prior to opening. Aliquot antibodies and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Epitope Tag kit contains: 6X HIS Antibody 100 µg DYKDDDDK Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) 250 µg GFP Antibody 100 µg GST Antibody 1.0mg Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated 100 µg Anti-GOAT IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated 100 µg
Purity/Specifity
6X HIS antibody, GFP Antibody, DYKDDDDK Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody), GST Antibody and Conjugated Secondary Antibodies are all affinity purified.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, IP
Catalog Number
600-101-200

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
wt and all variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP and EGFP
Catalog Number
600-401-215

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
600-401-382

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Reacts With
Carboxy and amino terminal linked FLAG™ tagged recombinant proteins
Catalog Number
600-401-383

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry