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Primary Antibodies  >  Transcription Factor Antibodies
 

NFkB Antibody Sampler Kit

Code

K711
Size

1 Kit
Price

$805.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Rockland produces kits to meet the requirements of researchers for highly sensitive chemiluminescence and other antibody based assays.
 Western blot of HeLa cell extract. A  predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed with anti NFkB antibody 100-4165.  All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.  Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h.  Washes with TBS preceded color development.
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Synonyms: Anti-NFKB p50, Anti-NFKB p65, Rabbit Anti-NFKB cRel, Anti-IKBalpha, Control peptides, antibody sample kits

NFkB Antibody Sampler Kit Properties

Description
NFKB ANTIBODY SAMPLER KIT - K711
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Product Type
Kit
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Label
Download CofA
Lot#  

NFkB Antibody Sampler Kit Description

Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Each kit contains one unit of the following products: Anti-NFKB p50 (NFKB1) (RABBIT) Antibody (100 µL) Anti-NFKB p65 (Rel A) (RABBIT) Antibody (100 µL) Anti-NFKB cRel (RABBIT) Antibody (100 µL) Anti-IKBa C-terminal (RABBIT) Antibody (100 µL) Anti-RABBIT IgG (H&L) (GOAT) Peroxidase Conjugated Antibody (100 µg) CONTROL PEPTIDE for Anti-NFKB p50 (NFKB1) (RABBIT) Antibody (50 µg) CONTROL PEPTIDE for Anti-NFKB p65 (Rel A) (RABBIT) Antibody (50 µg) CONTROL PEPTIDE for Anti-NFKB cRel (RABBIT) Antibody (50 µg) CONTROL PEPTIDE for Anti-IKBa C-terminal (RABBIT) Antibody ( (50 µg)
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-4164

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
100-4164P

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, GS, Other
Reacts With
human, mouse, rat
Catalog Number
100-4165

Product Type
Proteins and Peptides;
Reacts With
n/a
Catalog Number
100-4165P

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry