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Supporting Reagents  >  Blocking Solutions and BSA
 

Blocking Buffer Sampler Kit

NCI Collaboration
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Goat Serum blocking reagent or NGS is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.  This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications.
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Number:
K505
Size:
1 Kit
Price:
$159.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Blocking Buffer Sampler Kit Properties

Description
Blocking Buffer Sampler Kit - K505
Application
ELISA : User Optimized
IF Microscopy : User Optimized
Western Blot : User Optimized
Immunohistochemistry: User Optimized
Product Type
Solution
Product Type
Kit
Kit Type
Sampler Pack
Blocker Type
Other
Substrate/Enzyme Type
Blocking Reagent
Product Type
Blocking Reagent
Physical State
Liquid and Lyophilized
Shipping Condition
Wet Ice
Buffer
See application note.
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Blocking Buffer Sampler Kit Description

Background
Rockland lyophilized NORMAL GOAT SERUM (NGS) is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications, BOVINE SERUM ALBUMIN is suitable dilute solutions of antibody, use as a blocking agent to reduce non-specific binding, 2X Blocking Buffer for Fluorescent Western Blotting is optimized for Fluorescent Westen blotting and other immunoassays. ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free), 10X TBS Fish Gel Concentrate (Azide and Mercury free).
Storage Condition
Do not freeze kit. Store kit at 4° C. Kit contains multiple components. See kit insert for complete instructions.
Application Note
Blocking Buffer Sampler Kit contains: NORMAL GOAT SERUM (NGS), BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free), Blocking Buffer (2X) for Fluorescent Western Blotting, ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free) - Designed for ELISA assays, 10X TBS Fish Gel Concentrate (Azide and Mercury free) is a blocking agent for immunoassays and western blot.
Purity/Specifity
Lyophilized normal goat serum was prepared from normal goat serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. BSA has Purity (%): 100% by Agarose Zone Electrophoresis Protease: <0.005 units/mg by Enzymatic Assay. And no detectable IgG by Radial Immunodiffusion. Blocking Buffer (2X) for Fluorescent Western Blotting, ELISA Microwell blocking buffer with stabilizer, and Fish gel concentrate were aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The products were tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and found to be negative for bacteria.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Rabbit
Catalog Number
611-701-127

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-10

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Description
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Conjugation Reference
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Conjugation Chemistry