14-12_peptide top banner
 
Supporting Reagents  >  Enzyme Substrates
 

Chemiluminescent Femtomax™ Super Sensitive HRP Substrate For Microwell And/Or Membrane (2 Component Sytem)

NCI Collaboration
Order Offline
Western Blot of anti-GST tag antibody.  
Lane 1:  Recombinant GST tagged recombinant protein 5 ug.
Lane 2:  Recombinant GST tagged recombinant protein 2 ug.  
Lane 3:  Recombinant GST tagged recombinant protein 1 ug.  
Lane 4:  Recombinant GST tagged recombinant protein 500 ng.  
Lane 5:  Recombinant GST tagged recombinant protein 250 ng.  
Lane 6:  Recombinant GST tagged recombinant protein 100 ng.  
Lane 7:  Recombinant GST tagged recombinant protein 50 ng.  
Primary antibody:  anti-GST antibody at 1:1000 for overnight at 4°C.
Secondary antibody:  donkey secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  78 kDa.
View All Images
Number:
FEMTOMAX-020
Size:
20 mL
Price:
$99.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

Chemiluminescent Femtomax™ Super Sensitive HRP Substrate For Microwell And/Or Membrane (2 Component Sytem) Properties

Description
Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate for Microwell and/or Membrane (2 component sytem) - FEMTOMAX-020
Application
ELISA : 1X
Western Blot : 1X
Substrate/Enzyme Type
Substrate
Product Type
Substrate
Physical State
Liquid - clear, colorless, odorless
Shipping Condition
Ambient
Label
Download CofA:
Lot#  
Print

Chemiluminescent Femtomax™ Super Sensitive HRP Substrate For Microwell And/Or Membrane (2 Component Sytem) Description

Background
FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is an extremely sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP).  FemtoMax™ is designed for both western blotting and enzyme immunoassay (EIA) use.  FemtoMax™ easily allows for the detection of femtogram (10-15) amounts of antigen using photographic film or other imaging methods, including highly sensitive CCD cameras.  Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of detection reagents and re-probed. Use the same blotting conditions for FemtoMax™ as you would when using Amersham ECL Plus™ Substrate or Pierce SuperSignal® West Femto Substrate.
Storage Condition
Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.
Application Note
Prepare FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate for use in microwell or membrane applications by mixing 1 mL of Luminol Reagent (Reagent A) with 1 mL of Reaction Buffer (Reagent B).  Mix well.  Protect from light.  Larger or smaller volumes of the substrate can be prepared by mixing components at the same 1:1 ratio.  FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is a highly sensitive detection reagent.  Always carefully optimize all components of individual assays (antigens, antibodies, conjugates…) to minimize background reactivity associated with non-specific binding.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Log in to submit your product review.

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry