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Custom Products  >  Assay Development
 

Anti-Host Cell Protein

Code

CUST92C
Size

1 Each
Price

Price On Request
Availability

Ships within 2 weeks.
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Synonyms:

Anti-Host Cell Protein Properties

Description
Anti-Host Cell Protein (HCP) Antibody Production, Option A - CUST92C
Shipping condition
Ambient
Label
Download CofA
Lot#  

Anti-Host Cell Protein Description

Background
Complete service includes immunizing the HCP preparation after fractionation into high molecular weight (HMW) and low molecular weight (LMW) fractions by ultra filtration (provided by the client). Typically a membrane with a nominal molecular weight cutoff of 30,000 daltons is used to separate proteins less than 30kDa from those greater than 30 kDa. Separately HMW and LMW proteins are immunized into New Zealand White female rabbits. Six rabbits are immunized per immunogen. Project includes: 2 X 6 RABBIT hosts, 90 days daily maintenance, 12 Pre-bleeds (up to 5 mL each), 24 Test bleeds (up to 5 mL each), 20 production bleeds (4 production bleeds from the 5 best responding RABBITS) of up to 25 mL each or up to 500 mL). After a series of immunizations and test bleeds, reactivity against HCPs is assessed by western blot. Only host animals showing reactivity to a broad range of proteins by size are selected for production. Terminal bleeds (1 each from the best responding 5 RABBITS of 100 mL each or up to 500 mL), western blotting of all test bleeds, production bleeds and terminal bleeds and against one (1) HCP lysate provided by client. After selection, host animal production bleeds are pooled and then purified by a method equivalent to Protein A chromatography (7S fractionation or IgY purification from eggs) up to 1.0 L of qualified antiserum. Antibodies to HMW HCP proteins and antibodies to LMW HCP man be combined after purification and validation at the client's request. Vialing and lyophilization of up to 100 glass vials. Final qualification of final product by western blotting against provided HCP lysates. Summary report (1 page) with digital copy of finished product western blot and SDS-PAGE. Package charge for shipping of finished product is included.
Application Note
Complete service includes separation of the host cell protein into high molecular weight (HMW) and low molecular weight (LMW) fractions by ultra filtration, 10 RABBIT host animals, 90 days RABBIT daily maintenance, 10 RABBIT Pre-bleeds (5 mL each), 10 RABBIT test bleeds (5 mL each), 20 RABBIT production bleeds (4 production bleeds from the 5 best responding animals of 25 mL each or up to 500 mL), 5 Rabbit terminal bleeds (1 each from the 5 best responding animals of 100 mL each or up to 500 mL), western blotting of all test bleeds, production bleeds and terminal bleeds and against one (1) HCP lysate provided by client, Protein A purification of up to 1.0 L of qualified antiserum, vialing and lyophilization of up to 100 glass vials. Final qualification of final product by western blotting against provided HCP lysate. Summary report (1 page) with digital copy of finished product western blot and SDS-PAGE. Package charge for shipping of finished product is included.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry