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Avidin, Biotin, Streptavidin  >  Avidin-Biotin Reagents
 

Biotin Beta Galactosidase Conjugated

Code

B000-17
Size

1 mg
Price

$149.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Dot Blot of BIOTIN Conjugated BETA GALACTOSIDASE.
Lane 1:  BIOTIN Conjugated BETA GALACTOSIDASE.
Lane 2:  BETA GALACTOSIDASE.
Load:  3-fold serial dilution starting at 200 ng.
Primary Antibody:  None.
Secondary antibody:  HRP Streptavidin at 1:40,000 for 30 min at RT.
Block:  1% BSA-TTBS 30 min at RT.
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Vitamin H, Coenzyme R, Vitamin B7, ß-galactosidase, ß-gal, beta-gal

Biotin Beta Galactosidase Conjugated Properties

Description
BIOTIN BETA GALACTOSIDASE Conjugated - B000-17
Application
ELISA : 1:8,000 - 1:32,000
Western Blot : 1:500 - 1:2,500
Immunochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Reagent Type
Biotin
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.05 M Potassium Phosphate, 0.001M Magnesium Chloride, 0.1M Beta-Mercaptoethanol; pH 7.8
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Biotin Beta Galactosidase Conjugated Description

Background
Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. Beta-galactosidase (beta-gal; ß-gal) is an enzyme that hydrolyzes ß-galactosides (such as lactose and various glycoproteins) into simple sugars. Beta-galactosidase is routinely used as a reporter in biochemical experiments as well as a marker for gene expression experiments in E. coli. Beta-galactosidase can be used to cleave the chemical X-gal that forms an intense blue product that can easily be quantified.
Storage Condition
Store vial at 4° C before opening.   DO NOT FREEZE.   This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Freezing Beta Galactosidase conjugates will result in a substantial loss of enzymatic activity.   Expiration date is one (1) year from date of opening product.
Application Note
Biotin Beta Galactosidase Conjugated has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 is suggested for Biotin Beta Galactosidase Conjugated.
Purity/Specifity
Biotin Beta Galactosidase Conjugated was prepared from electrophoretically pure Beta Galactosidase and Biotin.   Biotin Beta Galactosidase assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Beta Galactosidase (E.coli) and anti-Biotin.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, GS, ChIP, Other
Catalog Number
A003-01

Product Type
Custom Products;
Catalog Number
CUST39

Product Type
Supporting Reagents;
Applications
WB, IHC
Catalog Number
IPTG-1000

Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, GS, ChIP, Other
Catalog Number
S000-01

Product Label

 

Description
Biotin
Color
n/a
Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
455
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester