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Avidin, Biotin, Streptavidin  >  Avidin-Biotin Reagents
 

Biotin Peroxidase Conjugated

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Dot Blot of Biotin Peroxidase Conjugated.  
Antigen:  Streptavidin.  
Load:  Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng.
Primary antibody:  n/a.
Secondary antibody:  Biotin Peroxidase Conjugated at 1:1,000 for 1 HR at RT.
Block:  MB-070 for 1 HR at RT.
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Number:
B000-03
Size:
5 mg
Price:
$105.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

Biotin Peroxidase Conjugated Properties

Description
BIOTIN PEROXIDASE Conjugated - B000-03
Application
ELISA : 1:20,000 - 1:200,000
Western Blot : 1:10,000 - 1:40,000
Immunohistochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Reagent Type
Biotin
Physical State
Lyophilized
Shipping Condition
Ambient
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
5.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Biotin Peroxidase Conjugated Description

Background
Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. Horse Raddish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Biotin Peroxidase Conjugated is ideal for investigators in Immunology, Cancer, Neuroscience, and Cell Biology.
Synonyms
Vitamin H, Coenzyme R, Vitamin B7, HRP, Horseradish peroxidase
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Biotin Peroxidase Conjugated is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Biotin Peroxidase Conjugated can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.
Purity/Specifity
Biotin Peroxidase Conjugated was prepared from chromatographically purified biotin. Biotin Peroxidase Conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Biotin.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
de Boer, Rodriguez, and Bonte et al. (2003). Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice. Proc Natl Acad Sci U S A. 2003 June 24; 100(13): 7480–7485. Veitch, Nigel C. (2004). Horseradish peroxidase: a modern view of a classic enzyme. Phytochemistry. Feb., Vol. 65. (3): 249-259.
Specific Reference
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Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, GS, ChIP, Other
Catalog Number
A003-01


Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-1000

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate