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Avidin, Biotin, Streptavidin  >  Avidin-Biotin Reagents
 

AVIDIN

Code

A003-01
Size

10 mg
Price

$59.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
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SDS-Page of Avidin.
Lane 1:  Avidin - reduced.  
Lane 2:  Avidin - non-reduced. 
Load:  1.0 µg per lane.
Predicted/Observed size:  16 kDa, 16 kDa for Avidin.
Other Band(s):  None.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: AVD

AVIDIN Properties

Description
AVIDIN - A003-01
Application
ELISA : User Optimized
ChIP : User Optimized
GelShift : User Optimized
Western Blot : User Optimized
Reagent Type
Avidin
Physical State
Lyophilized
Shipping condition
Ambient
Label
Concentration
10 mg/mL by dry weight
Specific Activity
14.4 U/mg by biotin titration method
Buffer
None
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
Related Links
Download CofA
Lot#  

AVIDIN Description

Background
Avidin is biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the greatest known non-covalent interactions. Avidity for biotin is destroyed with heat.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Avidin is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Avidin is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes; Southern blots and other methodologies related to DNA and RNA analysis; Western blots; and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized avidin.
Purity/Specifity
Avidin was prepared from chromatographically purified avidin isolated from egg white followed by extensive dialysis against the buffer stated above.  Avidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin.  No reaction was observed against anti-Streptavidin.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
100-4197

Product Type
GFP and Epitopes;
Applications
FLISA, WB, IF
Catalog Number
200-4297

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Mouse
Catalog Number
610-1619-0500

Product Type
Secondary Antibodies;
Reacts With
Rabbit
Catalog Number
611-106-B76

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry