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Secondary Antibodies  >  Fab Fragment Antibodies
 

Fab Mouse IgG (H&L) Antibody Peroxidase Conjugated

Goat Polyclonal
Code

810-1302
Size

1 mg
Price

$185.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western blot of Peroxidase conjugated Goat Fab Anti-Mouse IgG secondary antibody.
Lane 1:  Mouse IgG.
Lane 2:  None.
Load:  50 ng per lane.
Primary antibody:  None.
Secondary antibody:  Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.
Blocking:  MB-070 for 30 min at RT.
Predicted/Observed size:  25 & 55 kDa, 25 & 55 kDa for Mouse IgG.
Other band(s):  None.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Mouse IgG (H&L) Antibody, Gt-a-Mouse F(ab')2 Peroxidase conjugated, Mouse F(ab')2 Peroxidase Antibody in goat, Goat F(ab')2 peroxidase conjugated Secondary Antibody.

Fab Mouse IgG (H&L) Antibody Peroxidase Conjugated Properties

Description
Fab Anti-MOUSE IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 810-1302
Host
Goat
Specificity
IgG (H&L)
Target Species
Mouse
Known Cross Reactivity
Mouse
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:100,000
Western Blot : 1:2,000 - 1:20,000
Immunochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG Fab
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

Fab Mouse IgG (H&L) Antibody Peroxidase Conjugated Description

Background
Anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in goat detects specifically mouse IgG. This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Immunogen
Anti-Mouse IgG (H&L) was produced by repeated immunization with mouse IgG whole molecule in goat.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
Mouse IgG (H&L) peroxidase conjugated Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate