Fab Goat IgG (H&L) Antibody Peroxidase Conjugated

Datasheet

Fab Goat IgG (H&L) Antibody Peroxidase Conjugated

Name: Fab Goat IgG (H&L) Antibody Peroxidase Conjugated
Brand: Rockland Immunochemicals
Price: 292.0 USD
Availability: OutOfStock

Donkey Polyclonal

Item No. 805-7302

Size: 1 mg

Applications: ELISA

Reactivity: Goat

Host Species: Donkey

$292.00 /Per Item

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Availability: Ships on approximately 05/27/2024

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$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Fab Anti-Goat IgG Peroxidase Antibody generated in donkey detects goat IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Expand Background

Specifications for Fab Goat IgG (H&L) Antibody Peroxidase Conjugated

Product Details

Description: Donkey Fab Anti-Goat IgG (H&L) Antibody Peroxidase Conjugated - 805-7302

Synonyms: Donkey Fab Anti-Goat Antibody Peroxidase Conjugation, Donkey Fab Anti-Goat HRP Conjugated Antibody

Host Species: Donkey

Specificity: IgG (H&L)

Conjugate: Peroxidase (HRP)

Clonality: Polyclonal

Format: IgG Fab

Target Details

Reactivity: Goat

Immunogen Type: Native Protein

Immunogen: Anti-Goat IgG (H&L) was produced by repeated immunization with goat whole molecule in donkey.

Purity/Specificity: Fab Goat IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).

Application Details

Tested Applications: ELISA

Application Note: Anti-Goat IgG (H&L) peroxidase conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Formulation

Physical State: Lyophilized

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Related Protocols

ELISA Protocol

Sandwich ELISA Protocol for Collagen

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.