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Secondary Antibodies  >  F(ab')2 IgG Antibodies
 

F(ab')2 HUMAN IgG F(c) Pre-adsorbed Phycoerythrin Conjugated Antibody

Goat Polyclonal
Code

709-1817
Size

500 µg
Price

$269.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Western blot of Phycoerythrin conjugated Goat F(ab’)2 Anti-Human IgG F(c) Pre-Adsorbed secondary antibody.
Lane 1:  Human Fc.
Lane 2:  None.
Load:  50 ng per lane.
Primary antibody:  None.
Secondary antibody:  Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT.
Blocking:  MB-070 for 30 min at RT.
Predicted/Observed size:  28 kDa, 28 kDa for Human IgG F(c).
Other band(s):  None.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Human F(c) F(ab')2 Antibody, Gt-a-Human F(c) F(ab')2 phycoerythrin conjugated, Human F(c) F(ab')2 phycoerythrin Antibody in goat, Human F(c) F(ab')2 RPE conjugated Secondary Antibody.

F(ab')2 HUMAN IgG F(c) Pre-adsorbed Phycoerythrin Conjugated Antibody Properties

Description
F(ab')2 Anti-HUMAN IgG F(c) (GOAT) Antibody Phycoerythrin conjugated Min X Bv Hs Ms & Rt Serum Proteins - 709-1817
Host
Goat
Specificity
IgG F(c)
Target Species
Human
Known Cross Reactivity
Human
Clonality
Polyclonal
Application
IF Microscopy : 1:100 - 1:250
FlowCytometry : 1:100 - 1:250
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG F(ab')2
Concentration
0.5 mg/mL by absorbance = 82.0 at 565 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Download CofA
Lot#  

F(ab')2 HUMAN IgG F(c) Pre-adsorbed Phycoerythrin Conjugated Antibody Description

Background
F(ab')2 HUMAN IgG F(c) Pre-adsorbed Phycoerythrin Conjugated Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 HUMAN IgG F(c) Pre-adsorbed Phycoerythrin Conjugated Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
Immunogen
Anti-Human IgG was produced by repeated immunization with Human IgG F(c) fragment in goat.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Application Note
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab’)2 or Bovine, Horse and Mouse Serum Proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304


Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Avidin, Biotin, Streptavidin;
Applications
FLISA, WB, IF
Catalog Number
PA00-41

Product Label

 

Description
R-Phycoerythrin (RPE)
Color
Red
Conjugation Reference
Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversible protein-protein conjugation. Biochem J 173, 723-737 (1978).
Molecular Weight
240000
Excitation Wavelength
488
Conjugation Chemistry
SATA/MBS