House Secondary Banner
 
You may also want to consider these related products.
Anti-RAT IgG (H&L) (GOAT) Antibody - 112-1102
Anti-RAT IgG (H&L) (GOAT) Antibody - 112-1102
ABTS ELISA PEROXIDASE SUBSTRATE - ABTS-100
ABTS ELISA PEROXIDASE SUBSTRATE - ABTS-100
NORMAL GOAT SERUM (NGS) - B304
NORMAL GOAT SERUM (NGS) - B304
BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free) - BSA-50
BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free) - BSA-50
Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

Rat IgG (H&L) Antibody Biotin Conjugated

Goat Polyclonal
Code

612-1602
Size

2 mg
Price

$115.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western Blot of Goat anti-Rat IgG (H&L) Antibody Biotin Conjugated.
Lane 1:  Rat IgG.
Load:  100 ng per lane.
Primary antibody:  Rat IgG (H&L) Antibody Biotin Conjugated at 1:1000 for 60 min RT.
Secondary antibody:  HRP Conjugated Streptavidin at 1:40,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms:

Rat IgG (H&L) Antibody Biotin Conjugated Properties

Description
Anti-RAT IgG (H&L) (GOAT) Antibody Biotin Conjugated - 612-1602
Host
Goat
Specificity
IgG (H&L)
Target Species
Rat
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
2.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Download CofA
Lot#  

Rat IgG (H&L) Antibody Biotin Conjugated Description

Background
RAT IgG Biotin Conjugated antibody detects rat immunoglobulin G. Immunoglobuoin G is a molecule of about 150 kDa composed of four peptide chains. Each IgG contains two identical ? heavy chains of about 51 kDa and two identical light chains of about 26 kDa, thus a tetrameric quaternary structure. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape. Each end of the fork contains an identical antigen binding site. The Fc regions of IgGs bear a highly conserved N-glycosylation site.
Immunogen
Rat IgG whole molecule
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
Anti-RAT IgG Biotin Conjugated antibody is suitable for immunoassays where specificity to the Rat immunoglobulin heavy and or light chain regions is desired. Anti-Rat antibody has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:100,000 to 1:500,000 is suggested for this product. Optimal concentrations in immunoassays should be determined by the researcher.
Purity/Specifity
Anti-RAT IgG Biotin Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgG and Rat Serum.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Janeway CA, Jr. et al. (2001). Immunobiology. (5th ed.). Garland Publishing.
Specific Reference
Log in to submit your product review.

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
112-1102

Product Type
Supporting Reagents;
Applications
ELISA
Catalog Number
ABTS-100

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Biotin
Color
n/a
Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
455
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester