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Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

RABBIT IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Goat Polyclonal
Code

611-1322
Size

2 mg
Price

$135.00
Availability

Ships next business day.
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Western Blot of Goat anti-Rabbit IgG Peroxidase Conjugated Antibody.  
Lane 1:  HeLa Whole Cell Lysate.  
Lane 2:  NIH 3T3 Whole Cell Lysate.  
Load:  10 µg per lane.
Primary antibody:  Beta Actin antibody at 1:2,000 for overnight at 4°C.
Secondary antibody (2 Blots on Left):  Atto 647N goat secondary antibody at 1:10,000 for 60 min at RT.
Secondary Antibody (2 Blots on Right):  Peroxidase goat secondary antibody at 1:10,000 for 60 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  50 kDa, 50 kDa for Beta Actin. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Rabbit Antibody peroxidase conjugated, Gt-a-Rabbit peroxidase conjugated IgG, Rabbit proxidase conjugated Antibody in Goat, Rabbit Secondary peroxidase conjugated Antibody, HRP, peroxidase.

RABBIT IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed Properties

Description
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Human Serum Proteins) - 611-1322
Host
Goat
Specificity
IgG (H&L)
Target Species
Rabbit
Known Cross Reactivity
Rabbit
Clonality
Polyclonal
Application
ELISA : 1:150,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:750 - 1:2,500
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Concentration
2.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

RABBIT IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed Description

Background
Anti-Rabbit IgG peroxidase conjugated antibody generated in goat detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Immunogen
Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Purity/Specifity
RABBIT IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Human Serum Proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate