Mouse IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed
WB - Mouse IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed
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Datasheet

Mouse IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Donkey Polyclonal

2 References
610-706-124
1 mg
WB, ELISA, IHC
Mouse
Donkey
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Background

Mouse IgG antibody generated in donkey detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Product Details

Donkey Anti-Mouse IgG (H&L) Antibody Biotin Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) - 610-706-124
Donkey anti-Mouse IgG Biotin conjugated Antibody, Donkey anti-Mouse IgG Antibody Biotin conjugation
Donkey
IgG (H&L)
Biotin
Polyclonal
IgG

Target Details

Mouse
Native Protein
Mouse IgG whole molecule
Anti-Mouse IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Application Details

ELISA, WB
IHC  - View References
Anti-Mouse IgG Biotin conjugated antibody has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation

Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). Analysis of the regenerative capacity of human serum exosomes after a simple multistep separation from lipoproteins. J Tissue Eng Regen Med.
Applications
IHC, ICC, Histology
(). Mice lacking α1,3‐fucosyltransferase 9 exhibit modulation of in vivo immune responses against pathogens. Pathol Int.
Applications
IHC, ICC, Histology

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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