Background

Anti-Mouse IgG whole molecule antibody generated in sheep detects specifically Mouse IgG heavy and light chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Product Details

Sheep Anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated - 610-603-002
sheep anti-Mouse IgG heavy and light chain peroxidase conjugated Antibody, sheep anti-Mouse IgG HRP secondary Antibody, sh anti-ms IgG HRP antibody
Sheep
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG

Target Details

Mouse
Native Protein
Anti-Mouse IgG [H&L] was produced by repeated immunization with Mouse IgG whole molecule in sheep.
Sheep Anti-Mouse IgG[H&L] Peroxidase Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Mouse IgG and Mouse Serum.

Application Details

ELISA
WB  - View References
Anti-Mouse IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Formulation

Lyophilized
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). Novel Cross-Reactive Monoclonal Antibodies against Ebolavirus Glycoproteins Show Protection in a Murine Challenge Model. Journal of Virology
Applications
E, EIA
(). Development of an influenza virus protein microarray to measure the humoral response to influenza virus infection in mallards. Emerging Microbes & Infections
Applications
E, EIA
(). Rho GTPase techniques in osteoclastogenesis. Methods Mol Biol.
Applications
WB, IB, PCA
(). Sbds is required for Rac2-mediated monocyte migration and signaling downstream of RANK during osteoclastogenesis. Blood.
Applications
WB, IB, PCA
(). Filamin A regulates monocyte migration through Rho small GTPases during osteoclastogenesis. J Bone Miner Res.
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.