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Proteins and Peptides  >  Cytokines and other Growth Factors
 

Leptin Mouse Recombinant Protein

Other - E.coli
NCI Collaboration
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SDS-PAGE of Leptin Mouse Cytokine.  
Lane 1:  Leptin, reduced.  
Lane 2:  prestained MW markers.  
Load:  1 µg per lane.
Predicted/Observed size: 16 kDa, ~14 kDa for Leptin. 
Other band(s): none.
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Number:
010-001-320
Size:
1000 µg
Price:
$225.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

Leptin Mouse Recombinant Protein Properties

Description
Leptin Mouse Recombinant Protein - 010-001-320
Host
Other - E.coli
Known Cross Reactivity
Mouse
Application
Other Dilution: User Optimized
Species of Origin
Mouse
Physical State
Lyophilized
Shipping Condition
Ambient
PSC Type
Cytokine, Interleukin or Growth Factor
PSC Format
Recombinant Protein
Label
Concentration
0.1 mg/mL by UV absorbance at 280 nm
Buffer
0.1% Trifluoroacetic acid
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
Lep
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Leptin Mouse Recombinant Protein Description

Background
Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiologic regulation of body weight leptin has a variety of other physiologic and pathologic functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant Mouse Leptin produced in E. coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.1 kDa.
Synonyms
Leptin, LEP cytokine, Obesity factor, Obese protein
Storage Condition
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Application Note
Leptin is suitable as a control for polyclonal or monoclonal anti-Leptin in immunological assays.
Purity/Specifity
Purity was determined to be greater than 95% by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
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Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry