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Proteins and Peptides  >  Cytokines and other Growth Factors
 

IL-6 Mouse Recombinant Protein

Other - E.coli
Code

010-001-310
Size

10 µg
Price

$225.00
Availability

Ships within 2 weeks.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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SDS-PAGE using Rockland's Recombinant Mouse IL-6 Protein shows bands corresponding to IL-6  (1µg) in lane 1 (unreduced, arrowhead) and lane 3 (reduced).   Molecular weight estimation was made by comparison to prestained MW markers, lane 2.
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Interleukin-6 cytokine, IL-6, B-cell stimulatory factor 2, BSF-2, Interferon beta-2, IFN-beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, Interleukin HP-1

IL-6 Mouse Recombinant Protein Properties

Description
IL-6 Mouse Recombinant Protein - 010-001-310
Host
Other - E.coli
Known Cross Reactivity
Mouse
Application
Other Dilution: User Optimized
Species of Origin
Mouse
Physical State
Lyophilized
Shipping condition
Ambient
PSC Type
Cytokine, Interleukin or Growth Factor
PSC Format
Recombinant Protein
Label
Concentration
0.1 mg/mL by UV absorbance at 280 nm
Buffer
None
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
Il6
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Lot#  

IL-6 Mouse Recombinant Protein Description

Background
Interleukin (IL)-6, also know as BCDG, BCGF and BSF-2, is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Recombinant Mouse IL-6 produced in E. coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21,709 Daltons.
Storage Condition
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Application Note
Interleukin-6 (IL-6) is suitable as a control for polyclonal or monoclonal anti-Interleukin-6 (IL-6) in immunological assays. It is recommended to reconstitute the lyophilized recombinant M-IL-6 in 10 mM HCl to 0.1-1.0 mg/mL to regain full activity; this can then be further diluted to other aqueous solutions.
Purity/Specifity
Purity greater than 95% as determined by reducing and non-reducing SDS-PAGE.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Blankenstein T., Qin Z., Li W., Diamantstein T. (1990) DNA rearrangement and constitutive expression of the interleukin 6 gene in a mouse plasmacytoma. J. Exp. Med. 171:965-970 [PubMed: 2106569] [Abstract]
Specific Reference
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Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry