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Proteins and Peptides  >  Cytokines and other Growth Factors
 

IL-2 Human Recombinant Protein

Other - E.coli
Code

009-001-B95
Size

50 µg
Price

$225.00
Availability

Ships within 2 weeks.
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SDS-PAGE shows bands corresponding to IL-2  (1µg) in lane 1 (reduced) and lane 3 (non-reduced, arrowhead).   Molecular weight estimation was made by comparison to prestained MW markers, lane 2.
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$40.00 to United States
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Synonyms: Interleukin-2 cytokine, aldesleukin, T cell growth factor2, TCGF,

IL-2 Human Recombinant Protein Properties

Description
IL-2 Human Recombinant Protein - 009-001-B95
Host
Other - E.coli
Known Cross Reactivity
Human
Application
Other Dilution: User Optimized
Species of Origin
Human
Physical State
Lyophilized
Shipping condition
Ambient
PSC Type
Cytokine, Interleukin or Growth Factor
PSC Format
Recombinant Protein
Label
Concentration
0.1 mg/mL by UV absorbance at 280 nm
Buffer
0.01 M Sodium Citrate, pH 3.0
Reconstitution Volume
200 µL to 40 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
IL2
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IL-2 Human Recombinant Protein Description

Background
Interleukin-2 (IL2) is a secreted immunomodulatory cytokine that is essential in regulation and proliferation of T and B lymphocytes, and other activities crucial to regulation of the immune response. The receptor of this cytokine (the IL-2R receptor) is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells. Produced by T-cells in response to antigenic or mitogenic stimulation, this protein can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. Recombinant IL-2 produced in E. coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15,517 Daltons. Recombinant human IL-2 has a Ser substitute for Cysteine at position 126.
Storage Condition
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Application Note
IL-2 is suitable as a control for polyclonal or monoclonal anti-IL-2 in immunological assays.
Purity/Specifity
Purity was determined to be greater than 90% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS- PAGE, against known standard.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Weir,M.P., Chaplin,M.A., Wallace,D.M., Dykes,C.W. and Hobden,A.N. (1988) Structure-activity relationships of recombinant human interleukin 2 Biochemistry 27 (18), 6883-6892 PUBMED 3264184
Specific Reference
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Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry