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Proteins and Peptides > Cytokines and other Growth Factors

TNF-a Human Recombinant Protein

Other - E.coli

Code

009-001-306

Size

50 µg

Price

$215.00

Availability

Ships next business day.

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Phone: 800.656.7625

E-mail: orders@rockland-inc.com

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SDS-PAGE shows bands corresponding to TNFa (1µg) in lane 1 (unreduced, arrowhead) and lane 3 (reduced). Molecular weight estimation was made by comparison to prestained MW markers, lane 2.

Shipping & Handling Fees Apply.

Shipping:

$40.00 to United States

Handling:

$35.00

Synonyms: Tumor necrosis factor, TNF-alpha, TNF-a, TNFa, cytokine, Tumor necrosis factor ligand superfamily member 2, Cachectin

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TNF-a Human Recombinant Protein Properties

Description

TNF-a Human Recombinant Protein - 009-001-306

Host

Other - E.coli

Known Cross Reactivity

Human

Application

Other Dilution: User Optimized

Species of Origin

Human

Physical State

Lyophilized

Shipping condition

Ambient

PSC Type

Cytokine, Interleukin or Growth Factor

PSC Format

Recombinant Protein

Label

Unconjugated

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reconstitution Volume

500 µL

Reconstitution Buffer

Restore with deionized water (or equivalent)

Preservative

None

Stabilizer

None

GeneName

TNFA

TNF-a Human Recombinant Protein Description

Background

TNFa is secreted by macrophages, monocytes, neutrophils, T-cells, and NK- cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha, while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, and GM-CSF. Recombinant Human Tumor Necrosis Factor-alpha produced in E. coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17483.77 Daltons.

Storage Condition

Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Application Note

TNFa is suitable as a control for polyclonal or monoclonal anti-TNFa in immunological assays.

Purity/Specifity

Purity was determined to be greater than 98% by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

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