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GFP and Epitopes  >  Antibodies to GFP and Antibodies to RFP
 

Recombinant Red Fluorescent Protein (RFP) Control

Code

000-001-379
Size

100 µg
Price

$295.00
Availability

Ships next business day.
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SDS-PAGE of purified RFP control shows a single band approximately 28-30 kDa (arrow).  Approximately 1 µg of purified protein was loaded on to a 4-20% gradient gel for separation followed by staining with Coommassie blue. Molecular weight estimation was made by comparison to MW markers indicated at the right.
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Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Recombinant Red Fluorescent Protein (RFP) Control Properties

Description
Recombinant Red Fluorescent Protein (RFP) Control - 000-001-379
Application
Other Dilution: User Optimized
Epitope Tag Type
RFP
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
None
Stabilizer
None
GeneName
DsRed
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Lot#  

Recombinant Red Fluorescent Protein (RFP) Control Description

Background
Recombinant RFP (wild type, sequence corresponding to RFP, Discosoma spp.) is intended for use as a control when using polyclonal or monoclonal anti-RFP in immunological assays, in fluorometry, and in fluorescence activated cell sorting (FACS). Polyclonal anti-RFP can detect RFP by ELISA and western blot. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA is well suited to titrate RFP in solution when used in combination with unconjugated anti-RFP antibody. This product can be used as a control in western blots.
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
RFP is suitable as a control for polyclonal or monoclonal anti-RFP in immunological assays. The molecular weight of RFP (calculated) is 27 kDa, but in a gel it is expected to run at approximately 28-30 kDa.
Purity/Specifity
Recombinant RFP was synthesized with an amino terimal 6XHIS tag and was expressed in E.coli. A 3 step process was used to purify the protein: a partioning separation using t-butanol, followed by hydrophobic interaction chromatography (HIC), and lastly IMAC nickel chromatography. Analysis by SDS-PAGE resulted in a pattern consistent with purified RFP and was estimated to be greater than 95% pure.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Gross LA, Baird GS, Hoffman RC, Baldridge KK, Tsien RY: The structure of the chromophore within DsRed, a red fluorescent protein from coral. Proc. Natl. Acad. Sci. USA 2000, 97: 11990-11995.
Specific Reference
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Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, IP
Catalog Number
200-301-200

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IP
Catalog Number
200-301-382

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, IF, IP
Reacts With
wt, rGFP, eGFP
Catalog Number
600-101-215

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, IF
Catalog Number
600-401-379

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry