Rabbit IgG F(c) Antibody Peroxidase Conjugated Pre-Adsorbed
Western Blot of Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103)
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Datasheet

Rabbit IgG F(c) Antibody Peroxidase Conjugated Pre-Adsorbed

Goat Polyclonal

3 References
611-1323
2 mg
WB, ELISA
Rabbit
Goat
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Background

Anti-Rabbit IgG F(c) peroxidase antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

Product Details

Goat Anti-Rabbit IgG Fc Antibody Peroxidase Conjugated (Min X Human Serum Proteins) - 611-1323
Goat anti-Rabbit IgG F(c) Antibody peroxidase Conjugation, Goat anti-Rabbit IgG Fc fragment Antibody peroxidase Conjugation, Goat anti-Rabbit IgG F(c) HRP Conjugated Antibody
Goat
IgG Fc
Peroxidase (HRP)
Polyclonal
IgG

Target Details

Rabbit
Native Protein
Anti-Rabbit IgG was produced by repeated immunization with rabbit IgG F(c) fragment in goat.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab) or Human Serum Proteins.

Application Details

ELISA
WB  - View References
Antibody Anti-Rabbit IgG F(c) peroxidase conjugated has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Formulation

Lyophilized
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Characterization of MORN2 stability and regulatory function in LC3-associated phagocytosis in macrophages. Biol Open.
Applications
WB, IB, PCA
(). Cell adhesion controlled by adhesion G protein-coupled receptor GPR124/ADGRA2 is mediated by a protein complex comprising intersectins and Elmo-Dock. Journal of Biological Chemistry
Applications
WB, IB, PCA
(). The autophagic defect in Niemann–Pick disease type C neurons differs from somatic cells and reduces neuronal viability. Neurobiol Dis.
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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