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Secondary Antibodies  >  DyLight™ Conjugated Antibodies

Chicken IgG (H&L) Antibody Dylight™ 680 Conjugated

Goat Polyclonal


500 µg


Ships within 2 weeks.
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DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal.  Anti-tubulin was detected using a DyLight™ 549 conjugate.  Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
Western Blot of DyLight™ 680 conjugated Goat anti-Chicken IgG antibody.  
Lane 1:  Chicken IgG.  
Lane 2:  none.  
Load:  50 ng per lane.
Primary antibody:  none.
Secondary antibody:  DyLight™ 680 chicken secondary antibody at 1:5,000 for 60 min at RT.
Block: MB-070 for 30 min RT.
Predicted/Observed size:  72 kDa, 28 kDa for Chicken IgG. 
Other band(s): none.
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$40.00 to United States
Synonyms: DyLight Dye, DyLight Conjugated Antibody

Chicken IgG (H&L) Antibody Dylight™ 680 Conjugated Properties

Anti-Chicken IgG (H&L) (GOAT) Antibody DyLight™ 680 Conjugated (5 X 100 µg) - 603-144-002-0.5
IgG (H&L)
Target Species
Known Cross Reactivity
FLISA : >1:20,000
IF Microscopy : >1:5,000
Western Blot : >1:10,000
Other Dilution: User Optimized
Physical State
Shipping condition
FP Value
3.0 moles DyLight™ 680 per mole of IgG
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Chicken IgG (H&L) Antibody Dylight™ 680 Conjugated Description

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Chicken IgG, whole molecule
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum. This antibody will react with heavy chains of Chicken IgG and Chicken IgY and with light chains of most Chicken immunoglobulins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Label


DyLight™ 680
Near Infra-Red
Conjugation Reference
Modified from Thermo Fisher Scientific Inc, Rockford, IL
Molecular Weight
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester