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Secondary Antibodies  >  DyLight™ Conjugated Antibodies
 

Chicken IgG (H&L) Antibody Dylight™ 488 Conjugated

Goat Polyclonal
Code

603-141-002
Size

100 µg
Price

$85.00
Availability

Ships next business day.
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DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal.  Anti-tubulin was detected using a DyLight™ 549 conjugate.  Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
Western Blot of Goat anti-Chicken Antibody Dylight™ 488 Conjugated
Lane 1:  Chicken IgG
Load:  50 ng per lane
Secondary antibody:  Chicken IgG (H&L) Antibody Dylight™ 488 Conjugated at 1:1,000 for 60 min at RT
Block:  MB-070 for 30 min at RT
Predicted/Observed size:  72 and 28 kDa, 72 and 28 kDa
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Shipping:
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Synonyms: DyLight Dye, DyLight Conjugated Antibody

Chicken IgG (H&L) Antibody Dylight™ 488 Conjugated Properties

Description
Anti-Chicken IgG (H&L) (GOAT) Antibody DyLight™ 488 Conjugated - 603-141-002
Host
Goat
Specificity
IgG (H&L)
Target Species
Chicken
Known Cross Reactivity
Chicken
Clonality
Polyclonal
Application
FLISA : >1:20,000
IF Microscopy : >1:5,000
Western Blot : >1:10,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
FP Value
5.3 moles DyLight™ 488 per mole of IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Chicken IgG (H&L) Antibody Dylight™ 488 Conjugated Description

Background
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Immunogen
Chicken IgG, whole molecule
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum. This antibody will react with heavy chains of Chicken IgG and Chicken IgY and with light chains of most Chicken immunoglobulins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
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Product Label

 

Description
DyLight™ 488
Color
Green
Conjugation Reference
Modified from Thermo Fisher Scientific Inc, Rockford, IL
Molecular Weight
1011
Excitation Wavelength
493
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester