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Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

Chicken IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Goat Polyclonal
Code

603-105-126
Size

1 mg
Price

$215.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western Blot of Alkaline Phosphatase Conjugated Goat anti-Chicken IgG Pre-Adsorbed secondary antibody.
Lane 1:  Chicken IgG.
Lane 2:  None.
Load:  50 ng per lane.
Primary antibody:  None.
Secondary antibody:  Alkaline Phosphatase goat secondary antibody at 1:1,000 for 60 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  25 & 72 kDa, 25 & 72 kDa for Chicken IgG.
Other band(s):  None.
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Chicken Antibody, Gt-a-Chicken IgG, Chicken Antibody in Goat, Chicken Secondary Antibody

Chicken IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed Properties

Description
Anti-CHICKEN IgG (H&L) (GOAT) Antibody Alkaline Phosphatase Conjugated (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) - 603-105-126
Host
Goat
Specificity
IgG (H&L)
Target Species
Chicken
Known Cross Reactivity
Chicken
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:10,000
Western Blot : 1:500 - 1:2,500
Immunohistochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

Chicken IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed Description

Background
Anti-Chicken IgG whole molecule antibody generated in goat detects specifically Chicken IgG whole molecule. This secondary antibody anti-Chicken is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Immunogen
Anti-Chicken IgG whole molecule was produced by repeated immunization with Chicken IgG whole molecule in goat.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Application Note
Anti-Chicken IgG whole molecule is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum.   No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Alkaline Phosphatase (Calf Intestine)
Color
Substrate dependent
Conjugation Reference
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175, 1971.
Molecular Weight
140000
Excitation Wavelength
Conjugation Chemistry
Glutaraldehye coupling