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Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

Chicken IgG (H&L) Antibody

Goat Polyclonal
NCI Collaboration
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Western Blot of Anti-Chicken IgG (H&L) (GOAT) Antibody (p/n 603-101-002). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (GOAT) Antibody (p/n 603-101-002) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.
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Number:
603-105-002
Size:
1 mg
Price:
$159.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Chicken IgG (H&L) Antibody Properties

Description
Anti-CHICKEN IgG (H&L) (GOAT) Antibody Alkaline Phosphatase Conjugated - 603-105-002
Host
Goat
Specificity
IgG (H&L)
Target Species
Chicken
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:10,000
Western Blot : 1:500 - 1:2,500
Immunohistochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Chicken IgG (H&L) Antibody Description

Background
Anti-Chicken IgG (H&L) Antibody generated in goats detects specifically Chicken IgG whole molecule. Anti-Chicken IgG (H&L) is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Chicken IgG (H&L) Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Synonyms
ALP, Alk Phos
Immunogen
Chicken IgG whole molecule
Storage Condition
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Application Note
Anti-Chicken IgG (H&L) anitbody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.
Purity/Specifity
Anti-Chicken IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Chicken IgG and Chicken Serum.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Janeway, Jr., Travers, Walport, and Shlomchik. "The Immune System in Health and Disease." Immunobiology, 5th Edition: Garland Science: 2001.
Specific Reference
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Product Type
Secondary Antibodies;
Applications
FLISA, IF, FC
Catalog Number
603-100-002

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
603-105-007

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Chicken
Catalog Number
603-105-126

Product Type
Secondary Antibodies;
Applications
FLISA, WB, IF
Reacts With
Chicken
Catalog Number
603-141-002

Product Label

 

Description
Alkaline Phosphatase (Calf Intestine)
Color
Substrate dependent
Conjugation Reference
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175, 1971.
Molecular Weight
140000
Excitation Wavelength
Conjugation Chemistry
Glutaraldehye coupling