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Chicken IgG (H&L) Antibody Rhodamine Conjugated

Goat Polyclonal
NCI Collaboration
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Western Blot of Anti-Chicken IgG (H&L) (GOAT) Antibody (p/n 603-101-002). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (GOAT) Antibody (p/n 603-101-002) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.
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Number:
603-100-002
Size:
2 mg
Price:
$115.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

Chicken IgG (H&L) Antibody Rhodamine Conjugated Properties

Description
Anti-CHICKEN IgG (H&L) (GOAT) Antibody Rhodamine Conjugated - 603-100-002
Host
Goat
Specificity
IgG (H&L)
Target Species
Chicken
Clonality
Polyclonal
Application
FLISA : 1:10,000 - 1:50,000
IF Microscopy : 1:1,000 - 1:5,000
Flow Cytometry : 1:500 - 1:2,500
Other Dilution: FLOW CYTOMETRY 1:500 - 1:2,500
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
FP Value
2.2 moles Rhodamine (TRITC) per mole of IgG
Concentration
2.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Chicken IgG (H&L) Antibody Rhodamine Conjugated Description

Background
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Synonyms
TRITC
Immunogen
Chicken IgG whole molecule
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum..
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Rhodamine (TRITC)
Color
Red
Conjugation Reference
Modified from J.A. Titus, P.P. Haugland, S.D. Sharrow, D.M. Segal J. Immunol. Methods 50; 193, (1982).
Molecular Weight
444
Excitation Wavelength
550
Conjugation Chemistry
Isothiocyanate