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GFP and Epitopes  >  Antibodies to GST
 

GST Antibody Texas Red™ Conjugated

Rabbit Polyclonal
Code

600-409-200
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western Blot showing detection of recombinant GST protein (0.25 µg) in lane 2.  MW markers are in lane 1.  Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose.  After blocking with 1% BSA-TTBS (p/n  MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min.  HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system (Bio-Rad).
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Synonyms: GST, Glutathione-S-Transferase

GST Antibody Texas Red™ Conjugated Properties

Description
Anti-GST (RABBIT) Antibody Texas Red™ Conjugated - 600-409-200
Host
Rabbit
Clonality
Polyclonal
Application
ELISA : 1:60,000
IF Microscopy : User Optimized
Flow Cytometry : User Optimized
Western Blot : 1:2,000 - 1:10,000
Epitope Tag Type
GST
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
FP Value
4.8 moles Texas Red® per mole of IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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GST Antibody Texas Red™ Conjugated Description

Background
GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Immunogen
The immunogen is full length GST isolated from Schistosoma japonicum.
Immunogen Type
Native Protein
Storage Condition
Store Texas Red Conjugated Anti-GST antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  GST antibody is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Purity/Specifity
Texas Red Conjugated Anti-GST antibody was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].  Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Label

 

Description
Texas Red®
Color
Red
Conjugation Reference
Modified from J.A. Titus, P.P. Haugland, S.D. Sharrow, D.M. Segal J. Immunol. Methods 50; 193, (1982).
Molecular Weight
625
Excitation Wavelength
596
Conjugation Chemistry
Isothiocyanate