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COLLAGEN Type I Antibody Biotin Conjugated

Rabbit Polyclonal
NCI Collaboration
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Western Blot of Rabbit anti-Collagen I antibody.  
Lane 1:  Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced).  
Lane 2:  PPARg-transduced cell lysates.  
Load:  100 µg per lane. Protein staining shown below each blot depicts equal protein loading. 
Primary antibody:  anti-Collagen I antibody at 0.2–2 µg/10 ml for overnight at 4°C.
Secondary antibody: horseradish peroxidase-conjugated rabbit secondary antibody at 1 µg/10 ml for overnight at 4°C.
Block:  TBS with 5% Non-fat milk.
Predicted/Observed size: 138.9 kDa for Collagen I. 
Other band(s): none.
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Number:
600-406-103
Size:
100 µg
Price:
$309.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

COLLAGEN Type I Antibody Biotin Conjugated Properties

Description
Anti-Collagen Type I (RABBIT) Antibody Biotin Conjugated - 600-406-103
Host
Rabbit
Target Species
Mammalian
Known Cross Reactivity
human, bovine, rat, mouse
Clonality
Polyclonal
Application
ELISA : 1:3,000 - 1:6,000
Flow Cytometry : User Optimized
Western Blot : 1:3,000 - 1:6,000
Immunohistochemistry: 1:50 - 1:200
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
Label
FP Value
10-20 moles Biotin per mole of IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
GeneName
COL1A1
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COLLAGEN Type I Antibody Biotin Conjugated Description

Background
COLLAGEN Type I Antibody Biotin Conjugated is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.
Synonyms
Collagen Of Skin Tendon And Bone antibody, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen type I alpha 2 antibody, OI4 antibody, Osteogenesis Imperfecta Type IV antibody
Immunogen
Collagen Type I from human and bovine placenta.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.   Expiration date is one (1) year from date of restoration.
Application Note
Anti-COLLAGEN Type I Antibody Biotin Conjugated is suitable for western blot, immunoprecipitatioin, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
Purity/Specifity
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.  Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards.  Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.  This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V and VI collagens.  Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
001-001-103

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
009-001-103

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, mouse, rat, bovine
Catalog Number
600-401-103-0.5

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Biotin
Color
n/a
Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
455
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester