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Human Serum Albumin Antibody Biotin Conjugated

Rabbit Polyclonal
NCI Collaboration
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Western Blot of Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated.  
Lane 1:  Human Serum Albumin. 
Load:  50 ng per lane.
Primary antibody:  Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated at 1:1,000 overnight at 4°C.
Secondary antibody:  HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  66 kDa, 60 kDa for Human Serum Albumin.
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Number:
600-406-033
Size:
1 mg
Price:
$309.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

Human Serum Albumin Antibody Biotin Conjugated Properties

Description
Anti-HUMAN SERUM ALBUMIN (RABBIT) Antibody Biotin Conjugated - 600-406-033
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:60,000
Western Blot : 1:1,000 - 1:5,000
Immunohistochemistry: 1:500 - 1:3,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
Label
FP Value
10-20 moles Biotin per mole of IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/ml Polyethylene Glycol (PEG-8000)
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Human Serum Albumin Antibody Biotin Conjugated Description

Background
HUMAN SERUM ALBUMIN Antibody Biotin Conjugated, is the most abundant protein in human blood plasma. It is produced in the liver. Albumin constitutes about half of the blood serum protein. It is soluble and monomeric. Human serum albumin functions to maintain oncotic pressure, transports thyroid hormones, transport fat-soluble hormones, transport fatty acids to the liver, and transport unconjugated bilirubin. Anti-BSA antibody is suitable for immunological and cardiovascular research.
Synonyms
ALB antibody, Albumin antibody, Analbuminemia antibody, Bisalbuminemia antibody, Cell growth inhibiting protein 42 antibody, Dysalbuminemic hyperthyroxinemia antibody, Growth inhibiting protein 20 antibody, HSA antibody
Immunogen
Human Serum Albumin
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-HUMAN SERUM ALBUMIN (RABBIT) Antibody Biotin Conjugated antibody has been tested in ELISA, western blot, and immunohistochemical assays. Optimal concentrations should be determined by researcher.
Purity/Specifity
Anti-HUMAN SERUM ALBUMIN Antibody (Biotin Conjugated) was prepared from monospecific antiserum by immunoaffinity chromatography using purified human serum albumin.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.  Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Rothschild MA, Oratz M, Schreiber SS; Serum albumin. Hepatology. (1988) Mar-Apr;8(2):385-401.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-033

Product Type
Primary Antibodies;
Applications
FLISA, WB, IF
Reacts With
human
Catalog Number
600-402-033

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-403-033

Product Label

 

Description
Biotin
Color
n/a
Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
455
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester