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GFP and Epitopes  >  Antibodies to GST

GST Antibody Alkaline Phosphatase Conjugated

Rabbit Polyclonal
NCI Collaboration JCYIA Product
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100 µg
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GST Antibody Alkaline Phosphatase Conjugated Properties

Anti-GST (RABBIT) Antibody Alkaline Phosphatase Conjugated - 600-405-200
ELISA : 1:2,000-1:10,000
Western Blot : 1:500-1:2,500
Immunohistochemistry: 1:200-1:1,000
Other Dilution: User Optimized
Epitope Tag Type
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
0.5 mg/mL by UV absorbance at 280 nm
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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GST Antibody Alkaline Phosphatase Conjugated Description

GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
GST, Glutathione-S-Transferase, ALP, Alk Phos
The immunogen is full length GST isolated from Schistosoma japonicum.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Application Note
Suitable for ELISA, western blotting and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Alkaline Phosphatase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].  Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Label


Alkaline Phosphatase (Calf Intestine)
Substrate dependent
Conjugation Reference
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175, 1971.
Molecular Weight
Excitation Wavelength
Conjugation Chemistry
Glutaraldehye coupling