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Lysine Acetylated Antibody Peroxidase Conjugated

Rabbit Polyclonal
NCI Collaboration
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Rockland's Affinity Purified anti-Acetylated Lysine (AcK) antibody is shown to detect acetylated histone in TSA-treated mouse spleen cell lysate (Panel A); control (left lane) and TSA-treated mouse spleen cell lysate (right lane) in panel B; and in acetylated BSA loaded as indicated (panel C).
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Number:
600-403-939
Size:
100 µg
Price:
$355.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Lysine Acetylated Antibody Peroxidase Conjugated Properties

Description
Anti-Lysine Acetylated (AcK) (RABBIT) Antibody Peroxidase Conjugated - 600-403-939
Host
Rabbit
Known Cross Reactivity
mammalian, avian
Clonality
Polyclonal
Application
ELISA : 1:5000
Western Blot : 1:1000-1:5000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
IgG
Concentration
1 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
None
Stabilizer
50% (v/v) Glycerol
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Lysine Acetylated Antibody Peroxidase Conjugated Description

Background
Anti-Lysine Acetylated Antibody Peroxidase Conjugated is ideal for Cancer, Cell Biology and Chromatin & Nuclear Signaling research.
Synonyms
acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with acetylated lysine conjugated KLH.
Immunogen Type
Other
Storage Condition
Product is stable for several weeks at 4° C.  Dilute only prior to immediate use.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Application Note
This affinity-purified antibody is suitable for use in ELISA and western blotting assays.  The antibody reacts specifically with acetylated lysine residues.  Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using acetylated lysine peptide coupled to agarose followed by conjugation to horseradish peroxidase and subsequent purification.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG  and anti-Peroxidase.  Reactivity is specific for acetylated lysine.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, IP, FC
Reacts With
mammalian, avian
Catalog Number
600-401-939

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate