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Primary Antibodies  >  Serum Protein Component Antibodies
 

Human Serum Albumin Antibody Peroxidase Conjugated

Rabbit Polyclonal
Code

600-403-033
Size

1 mg
Price

$309.00
Availability

Ships next business day.
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Western Blot of human serum albumin.  
Lane 1:  1:400 of FBS.  
Lane 2:  1:1600 of FBS. 
Lane 3:  1:400 of normal rabbit serum.  
Lane 4:  1:1600 of normal rabbit serum. 
Lane 5:  1:400 of normal human serum.  
Lane 6:  1:1600 of normal human serum.   
Lane 7:  1:10 of human serum albumin (10mg/mL).  
Lane 8:  1:40 of human serum albumin (10mg/mL).  
Lane 9:  molecular weight.  
Right Blot: Secondary only incubated for control.  
Primary antibody:  human serum albumin at 1:400 for overnight at 4°C.
Secondary antibody: Biotin conjugated rabbit anti human serum albumin with 10 mg/ml BSA added to stock was diluted 1:5000 in PBST for 2hrs at RT.  Dylight 649 conjugated Stretavidin 1:50000 in PBST 1 hr RT was used as a secondary reagent.
Block:  1.5 % rabbit serum overnight at 4°C.
Western blot of Peroxidase conjugated Rabbit Anti-Human Serum Albumin secondary antibody.
Lane 1:  Human Serum Albumin (HSA).
Lane 2:  None.
Load:  50 ng per lane.
Primary antibody:  None.
Secondary antibody:  Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted\Observed size:  66 kDa, 61 kDa for HSA.
Other band(s):  None.
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Synonyms: ALB antibody, Albumin antibody, Analbuminemia antibody, Bisalbuminemia antibody, Cell growth inhibiting protein 42 antibody, Dysalbuminemic hyperthyroxinemia antibody, Growth inhibiting protein 20 antibody, HSA antibody

Human Serum Albumin Antibody Peroxidase Conjugated Properties

Description
Anti-HUMAN SERUM ALBUMIN (RABBIT) Antibody Peroxidase Conjugated - 600-403-033
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:1,000 - 1:5,000
Immunochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/ml Polyethylene Glycol (PEG-8000)
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Human Serum Albumin Antibody Peroxidase Conjugated Description

Background
Anti-Human Serum Albumin antibody detects Serum allbumin protein. Serum albumin is the main protein of plasma. It has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. ALB is the major zinc transporter in plasma; it typically binds about 80% of all plasma zinc. BSA antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
Immunogen
Anti-Human Serum Albumin Antibody was produced by repeated immunizations with Human Serum Albumin.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-ALB antibody is suitable for western blotting. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified human serum albumin IgG, and human Serum.
Purity/Specifity
Human Serum Albumin antibody was purified from pooled rabbit sera obtained from rabbits immunized with Human Serum Albumin.  Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification.  The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated above.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-033

Product Type
Primary Antibodies;
Applications
FLISA, WB, IF
Reacts With
human
Catalog Number
600-402-033

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-406-033

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate