House Phospho Banner
 
You may also want to consider these related products.
Anti-p53 (MOUSE) Monoclonal Antibody - 200-301-174
Anti-p53 (MOUSE) Monoclonal Antibody - 200-301-174
Anti-MDM2 pS185 (RABBIT) Antibody - 600-401-423
Anti-MDM2 pS185 (RABBIT) Antibody - 600-401-423
Anti-MDM2 (RABBIT) Antibody - 600-401-927
Anti-MDM2 (RABBIT) Antibody - 600-401-927
NORMAL GOAT SERUM (NGS) - B304
NORMAL GOAT SERUM (NGS) - B304
Primary Antibodies  >  Cancer Research Antibodies
 

ARF-BP1 Antibody

Rabbit Polyclonal
Code

600-401-C03
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Immunocytochemistry demonstrating detection of ARF-BP1 in Daudi cells.  The primary antibody was used at 5 µg/ml.
Western blot showing detection of ARF-BP1 in Daudi cell lysate.  Primary antibody was used at 1 µg/ml.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: ARF-BP1, ARF binding protein 1; HECT, UBA and WWE domain containing 1; HUWE1; Mcl-1 ubiquitin ligase E3; Mule; E3 ubiquitin-protein ligase HUWE1, KIAA0312, KIAA1578, UREB1

ARF-BP1 Antibody Properties

Description
Anti-ARF-BP1 (Rabbit) Antibody - 600-401-C03
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:40,000
IF Microscopy : 5.0 µg/mL
Western Blot : 1.0 -2.0 µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
HUWE1
Download CofA
Lot#  

ARF-BP1 Antibody Description

Background
The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents. Anti-ARF-BP1 antibody is ideal for investigators involved in cancer research.
Immunogen
ARF-BP1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human ARF-BP1 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
ARF-BP1 antibody has been tested in ELISA, WB, and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~481 kDa in size corresponding to ARF-BP1 by Western blotting in the appropriate cell lysate or extract. Daudi cell lysate can be used as positive control.
Purity/Specifity
Anti-ARF-BP1 Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with ARF-BP1 with Human, Rat and Mouse based on 100% homology with the immunizing sequence. Cross-reactivity with ARF-BP1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Gallagher SJ, Kefford RF and Rizos H. (2006) The ARF tumour suppressor. Int. J. Biochem. Cell Biol. 38:1637-41. Pomerantz J, Schreiber-Agus N, Leigeois NJ ( 1998) The Ink4a tumor suppressor gene product, p19ARF interacts with MDM2 and neutralizes MDM2’s inhibition of p53. Cell 92:725-34. Chen D, Kon N, Li M (2005) ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor. Cell 121:1071-83. Zhong Q, Gao W, Du F (2005) Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis. Cell 121:1085-95.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human
Catalog Number
200-301-174

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
600-401-423

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse
Catalog Number
600-401-927

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry