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Primary Antibodies  >  Sample Size Primary Antibodies
 

CASZ1 Antibody

Rabbit Polyclonal
Code

600-401-B62S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
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Western blot using Rockland's anti-hCASZ1 antibody.  This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 µg).   Protein was resolved by SDS-PAGE and transferred onto nitrocellulose.   After blocking, the membrane was probed with the primary antibody diluted to 1:1,000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature.  Lane 1, BE2(s) cell lysate.  Lane 2, BE2(N) cell lysate.  Lane 3, SY5Y transfected with hCas5 (125kDa).  Lane 4, SY5Y transfected with hCas11 (190kDa).  Personal communication, Carol Thiele, NCI, Bethesda, MD
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Synonyms: CASZ1, Zinc finger protein castor homolog 1, Castor-related protein, Zinc finger protein 693

CASZ1 Antibody Properties

Description
Anti-CASZ1 (RABBIT) Antibody - 600-401-B62S
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, Drosophila, chimpanzee, and macaque
Clonality
Polyclonal
Application
ELISA : 1:45,000
Western Blot : 1:1,000 - 1:5,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.09 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CASZ1
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Lot#  

CASZ1 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The human castor zinc finger 1 gene (CASZ1) regulates the production of the castor transcription factor, having increased expression when cells of neural and mesenchymal origin are induced to differentiation. CASZ1 is expressed in a number of human tumors and it localizes to a genomic region frequently lost in tumors of neuroectodermal origin. The CASZ1 gene generates 2 mRNA isoforms hCasz5 and hCasz11 that could encode proteins containing 5 or 11 zinc finger motifs, respectively. The proteins may be especially labile. Expect a band at 125 kDa for hCasz5 and 190 kDa for hCaz11 in fresh lysates.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human Casz1 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This antibody has been tested for use in ELISA and western blotting. This antiserum detects endogenous Casz1 proteins, both the hCasz5 and hCasz11 isoforms. Expect a band approximately 125 kDa for hCasz5 and 190 kDa for hCaz11 in size corresponding Casz1 by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
This affinity purified antibody is directed against human Casz1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with CASZ1 proteins from human, mouse, Drosophila, chimpanzee, and macaque based on a 100% homology. Partial reactivity is expected with horse and dog CASZ1 based on a 92% homology with the immunizing sequence. Cross-reactivity with CASZ1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Z Liu, X Yang, K Cullion and CJ Thiele, (2006) Molecular cloning and characterization of human castor (CASZ1), a novel human gene upregulated during cell differentiation. Biochemical and Biphysical Research Communications Volume 344(3): 834.
Specific Reference
Z Liu, A Naranjo, and CJ Thiele. (2011) CASZ1b, the Short Isoform of CASZ1 Gene, Coexpresses with CASZ1a during Neurogenesis and Suppresses Neuroblastoma Cell Growth. PLOS.ONE. April 07. DOI: 10.1371/journal.pone.0018557.
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, Drosophila, chimpanzee, and macaque
Catalog Number
600-401-B62

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Rabbit
Catalog Number
611-1322

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry