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Primary Antibodies  >  Sample Size Primary Antibodies
 

PRDM1 BLIMP1 Antibody

Rabbit Polyclonal
Code

600-401-B52S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
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Western blots using Rockland's affinity purified anti-PRDM1/BLIMP1 antibody show detection of overexpressed PRDM1/BLIMP1 in whole transfected Raji cell lysate (lane 2) at ~88kDa.  Lane 1 shows mock transfection in whole Raji cell lysate.   Detection of endogenous PRDM1/BLIMP1 (lane 4) is illustrated in human plasma cell nuclear extract, but not in Raji whole cell nuclear extract (lane 3).  The identity of the lower dark band at ~50-60kDa is unknown.   Primary antibody was used at a 1:1000 dilution in 5% PBS-Tween.   Personal communication, Hyesuk Yoon and Jerry Boss, Emory University, Atlanta, GA
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Synonyms: PR domain zinc finger protein 1, PR domain-containing protein 1, Beta-interferon gene positive regulatory domain I-binding factor, BLIMP-1, Beta-lymphocyte-induced maturation protein 1

PRDM1 BLIMP1 Antibody Properties

Description
Anti-PRDM1/Blimp1 (RABBIT) Antibody - 600-401-B52S
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat, dog
Clonality
Polyclonal
Application
ELISA : 1:65,000 - 1:80,000
Western Blot : 1:1,000
Immunochemistry: 5µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.15 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
PRDM1
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Lot#  

PRDM1 BLIMP1 Antibody Description

Background
PRDM1/Blimp1 (PR domain zinc finger protein 1, PR domain-containing protein 1, Beta-interferon gene positive regulatory domain I-binding factor, BLIMP-1, Positive regulatory domain I-binding factor 1, PRDI-binding factor 1, or PRDI-BF1, or Beta-lymphocyte-induced maturation protein 1) is one of several transcription factors which drives the maturation of B-lymphocytes into Ig secreting cells. In the nucleus, PRDM1/BLIMP1 functions as a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Transcription of the PRDM1 gene is increased upon virus induction. PRDM1/Blimp1 also interacts with PRMT5. Two alternatively spliced transcript variants that encode different isoforms have been reported.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of human, mouse, and rat PRDM1/BLIMP1.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~88 kDa in size corresponding to BLIMP1 by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity purified antibody is directed against human BLIMP1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is predicted to react with BLIMP1 and its isoforms from human, mouse, rat and other sources based on a 100% homology with the immunizing sequence. This antibody may also recognize other proteins that show high homology/identity with the BLIMP1 sequence.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Morris AC, Beresford GW, Mooney MR, and Boss JM. (2002) Kinetics of a Gamma Interferon Response: Expression and Assembly of CIITA Promoter IV and Inhibition by Methylation Mol Cell Biol, 22 (13) 4781-4791. Fujita, N., D.L. Jaye, C. Geigerman, A. Akyildiz, M.R. Mooney, J.M. Boss, and P.A. Wade. 2004. MTA3 and the Mi-2/NuRD complex regulate cell fate during B lymphocyte differentiation. Cell. 119:75–86. [PubMed] Nagy M., Chapuis B., Matthes T. (2002) Expression of transcription factors Pu.1, Spi-B, Blimp-1, BSAP and oct-2 in normal human plasma cells and in multiple myeloma cells.; Br. J. Haematol. 116:429-435(2002).. DOI=10.1046/j.1365-2141.2002.03271.x; PubMed=11841448 [NCBI, ExPASy, EBI, Israel, Japan]
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, mouse, rat, dog
Catalog Number
600-401-B52

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304


Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry