House Buffer
 
You may also want to consider these related products.
Anti-AKT pS473 (MOUSE) Monoclonal Antibody - 200-301-268
Anti-AKT pS473 (MOUSE) Monoclonal Antibody - 200-301-268
Anti-MOUSE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated - 610-4302
Anti-MOUSE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated - 610-4302
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
NORMAL GOAT SERUM (NGS) - B304
NORMAL GOAT SERUM (NGS) - B304
Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

TRAF2 Antibody

Rabbit Polyclonal
Code

600-401-B27
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western blot using Rockland's affinity purified anti-TRAF2 antibody shows detection of endogenous TRAF2 in whole HeLa cell lysates.  Lane 2 shows endogenous TRAF2 detected with antibody at 47 kDa (arrowhead).  Lane 4 shows no reactivity when blot is incubated with immunizing peptide. The identity of lower molecular weight band in lane 2 is unknown.  Briefly, each lane contains approximately 14 µg of lysate. Membranes were blocked in 3% BSA-TBS 30 min. at room temperature. Primary antibody was used at a 1:500 dilution in 3% BSA-TBS and reacted overnight at 4°C. The membrane was washed and reacted with a 1:20,000 dilution conjugated Gt-a-Rabbit DyLight 649 (611-143-122) for 1 hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers in lanes 1 and 3. Fluorescence image was captured using the VersaDoc® Imaging System developed by Bio-Rad.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: MGC:45012 antibody, TNF receptor associated factor 2 antibody, TRAF 2 antibody, TRAP 3 antibody, TRAP antibody, TRAP3 antibody, Tumor necrosis factor type 2 receptor associated protein 3 antibody

TRAF2 Antibody Properties

Description
Anti-TRAF2 (RABBIT) Antibody - 600-401-B27
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse
Clonality
Polyclonal
Application
ELISA : 1:60,000 - 1:250,000
IF Microscopy : user optimized
Western Blot : 1 µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.93 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
TRAF2
Download CofA
Lot#  

TRAF2 Antibody Description

Background
TRAF2, or Tumor Necrosis factor (TNF) Receptor-Associated Factor 2, is an adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Association to the receptor is also mediated by the interaction with TRADD. TRAF2 mediates activation of NF-kappa-B and MAPK8/JNK and is involved in apoptosis. TRAF2 forms a heterodimeric complex with TRAF1, which then recruits the inhibitor-of-apoptosis proteins (IAPs), apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2 for the inhibition of caspase activation. In this way it functions as a mediator of the anti-apoptotic signals from TNF receptors. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. TRAF2 may be involved in IL-15 signaling. Multiple alternatively spliced transcript variants exist, but the biological validity of only one transcript has been determined.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region human TRAF2.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Purity/Specifity
This affinity purified antibody is directed against human TRAF2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is predicted to react with TRAF2 from human and mouse sources based on a 100% homology with the immunizing sequence. Reactivity with TRAF2 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Li S., Wang L., Berman M., Zhang Y., and Dorf M. (2006) RNAi Screen in Mouse Astrocytes Identifies Phosphatases that Regulate NF-?B Signaling. Mol Cell. Nov 17; 24:497-509.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry