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Primary Antibodies  >  Sample Size Primary Antibodies
 

Lac I Antibody

Rabbit Polyclonal
Code

600-401-B04S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
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Western blot using Rockland's affinity purified anti-Lac I antibody shows detection of a 38 kDa band corresponding to recombinant Lac I (arrowhead).  The blot was blocked with 5% BLOTTO in TTBS overnight at 4°C.  Primary antibody was used at a 1:1,000 dilution followed by reaction with a 1:10,000 dilution of HRP goat anti-rabbit IgG.  ECL was used for detection. Personal communication, S. Hughes & M. Abram, NCI, Bethesda, MD.
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Synonyms: DNA binding transcriptional repressor antibody, LacI antibody, Transcriptional repressor of the lac operon antibody

Lac I Antibody Properties

Description
Anti-Lac I (RABBIT) Antibody - 600-401-B04S
Host
Rabbit
Target Species
Escherichia coli
Known Cross Reactivity
E.coli and S.sonnei
Clonality
Polyclonal
Application
ELISA : 1:90,000 – 1:100,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
LAC1
Download CofA
Lot#  

Lac I Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase; modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant Lac I protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein.   Specific conditions for reactivity and detection of Lac I should be optimized by the end user.  Expect a band approximately ~38 kDa in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with recombinant Lac I protein.  A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence.  Cross-reactivity with Lac I from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Oh J., Julias, JG, Ferris AL, and Hughes SH. (2002) Construction and characterization of a replication-competent retroviral shuttle vector plasmid. J. Virol 76(4):1762 Oh J, Chang KW, and Hughes SH (2006) Mutations in the U5 Sequences Adjacent to the Primer Binding Site Do Not Affect tRNA Cleavage by Rous Sarcoma Virus RNase H but Do Cause Aberrant Integrations In Vivo J.Virol 80(1):451 Sams CF, Hemelt VB, Pinkertone FD, Schroepfer GJ, Matthews KS (1985) Exposure of antigenic sites during immunization. Monoclonal antibodies to monomer of lactose repressor protein J Biol. Chem. 260(2):1185-1190
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
E.coli and S.sonnei
Catalog Number
600-401-B04

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Kits;
Applications
ELISA, WB, IHC, IF, IP
Catalog Number
K-500

Product Type
Supporting Reagents;
Applications
ELISA, WB, IHC, IF
Catalog Number
K505

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry