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Primary Antibodies > Sample Size Primary Antibodies

Oct-4 Antibody

Rabbit Polyclonal

Code

600-401-A95S

Size

25 µL

Price

$89.00

Availability

Ships next business day.

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Western blot using Rockland's affinity purified anti-Oct4 antibody shows detection of endogenous Oct4 in mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2). The band at ~39 kDa (arrow head) corresponds to Oct4. After transfer, the membrane was blocked with 5% BSA. Primary antibody was used at a 1:1,000 dilution in PBS containing 5% BSA. The specificity of the antibody was confirmed by peptide competition which blocks reaction of the antibody with Oct4 (lanes 3 and 4, respectively).

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Synonyms: Octamer binding protein 4 antibody, Octamer binding transcription factor 3 antibody, OTF3 antibody, OTF4 antibody, POU class 5 homeobox 1 antibody, POU domain class 5 transcription factor 1 antibody

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Oct-4 Antibody Properties

Description

Anti-Oct-4 (RABBIT) Antibody - 600-401-A95S

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human, mouse, monkey, horse

Clonality

Polyclonal

Application

ELISA : 1:350,000
IF Microscopy : User Optimized
Western Blot : 1:1,000 - 1:1,500
ImmunoPrecipitation: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

POU5F1

Oct-4 Antibody Description

Background

Oct-4, also known as POU domain transcription factor, Octomer- binding transcription factor 3 and Oct-3, is expressed in human and mouse totipotent embryonic stem and germ cells. It may function in preventing these cells from differentiating. Each type of established mammalian pluripotent stem cell line (embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells) expresses Oct-4, which disappears rapidly when the cells differentiate. Most studies on Oct-4 have been performed on murine embryos. In human oocytes of various maturity and normally developing preimplantation/cleavage embryos, a variable Oct-4 expression pattern has been revealed, concomitant with a pure cytoplasmic localization of the protein. This variability in expression fades away during compaction, indicating embryonic Oct-4 expression; the protein appears in the nucleus, implying biological activity. Functional Oct-4 has been found in human amniotic fluid. As Oct-4 transcripts have also been found in various differentiated cells, the presence of Oct-4 transcripts or proteins may not be sufficient for identifying undifferentiated cell lines in humans. It may also be important to examine the localization of Oct-4 proteins within a cell. Oct-4 has been shown to interact with Nanog and with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes, possibly to control ES fate. Reactivation of Oct-4 expression is postulated to occur in differentiated cells that have undergone carcinogenesis, or tumor formation.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Oct-4 protein.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting.

Purity/Specifity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human and mouse Oct-4 protein. Cross-reactivity with Oct-4 from other species has not been tested. A BLAST analysis was used to suggest additional cross-reactivity with Oct-4 from chimpanzee, macaque and horse based on a 100% homology with the immunizing sequence; and mink, dog, bovine and pig based on a 93% homology.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Prusa,A-R, Marton,E, Rosner,M, Bernaschek,G, and Hengstschläger,M. (2003) Oct-4-expressing cells in human amniotic fluid: a new source for stem cell research? Human Reproduction 18 (7), 1489-1493 Cauffman,G., Van de Velde,H., Liebaers,I., and Van Steirteghem,A. (2005) Oct-4 mRNA and protein expression during human preimplantation development Molecular Human Reproduction 11(3),173-181 Donovan, P.J. (2001) High Oct-ane fuel powers the stem cell. Nature Genet., 29, 246–247 Pesce, M. and Schöler, H.R. (2001) Oct-4: gatekeeper in the beginnings of mammalian development. Stem Cells, 19 (4), 271–278. Lin,L., Czerwiec,E., Keefe,D.L. (2004) Effect of ploidy and parental genome composition on expression of Oct-4 protein in mouse embryos. Gene Expression Patterns 4 (4), 433-441

Specific Reference

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