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Primary Antibodies > Cancer Research Antibodies

Eif3S6 Int6 Antibody

Rabbit Polyclonal

Code

600-401-A62

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000. Personal communication, J.Lee, NCI, Bethesda, MD.

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Synonyms: eIFe antibody, Eukaryotic translation initiation factor 3 subunit 6 antibody, INT6 antibody, Viral integration site protein INT-6 homolog antibody

Datasheet
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Eif3S6 Int6 Antibody Properties

Description

Anti-eIF3S6/Int6 (RABBIT) Antibody - 600-401-A62

Host

Rabbit

Target Species

Mouse

Known Cross Reactivity

mouse, human, rat, dog, bovine, monkey

Clonality

Polyclonal

Application

ELISA : 1:90,000
Western Blot : 1:1,000
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

0.8 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

EIF3E

Eif3S6 Int6 Antibody Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stress-responsive genes. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of mouse EIF3S6/Int6.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to eIF3S6/Int6 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both over-expressed and endogenous eIF3S6/Int6.

Purity/Specifity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for mouse eIF3S6/Int6 protein. A BLAST analysis was used to suggest cross-reactivity with most eIF3Se/Int6 isoforms from mouse, human, rat, dog, bovine, and monkey based on 100% homology with the immunizing sequence. Cross-reactivity with EIF3S6/Int6 from other sources has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Buttitta F, Martella C, Barassi F, Felicioni L, Salvatore S, Rosini S, D'Antuono T, Chella A, Mucilli F, Sacco R, Mezzetti A, Cuccurullo F, Callahan R, Marchetti A. (2005) Int6 expression can predict survival in early-stage non-small cell lung cancer patients. Clin Cancer Res. 11(9):3198-204. Lowther,W., Wiley,K., Smith,G.H. and Callahan,R. (2005) A new common integration site, Int7, for the mouse mammary tumor virus in mouse mammary tumors identifies a gene whose product has furin-like and thrombospondin-like sequences. J. Virol. 79 (15), 10093-10096. Bandyopadhyay,A., Matsumoto,T. and Maitra,U. (2000) Fission yeast Int6 is not essential for global translation initiation, but deletion of int6(+) causes hypersensitivity to caffeine and affects spore formation. Mol. Biol. Cell 11 (11), 4005-4018.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra