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Primary Antibodies > Transcription Factor Antibodies

SMAD4 Antibody

Rabbit Polyclonal

Code

600-401-A41

Size

100 µg

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's affinity purified anti-SMAD4 to detect over-expressed SMAD4 in transfected COS cells (lane 2). Lane 1 contains lysate from mock transfected COS cells. A doublet band is seen in the SMAD4 transfected lysate with the upper band (arrowhead) representing SMAD4 and the lower band being non-specific staining. The membrane was probed with the primary antibody at a 1:1,000 dilution. Personal Communication Kathleen Flanders, CCR-NCI, Bethesda, MD.

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Synonyms: Deletion target in pancreatic carcinoma 4 antibody, DPC 4 antibody, MAD mothers against decapentaplegic homolog 4 antibody, MADH 4 antibody

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SMAD4 Antibody Properties

Description

Anti-SMAD4 (RABBIT) Antibody - 600-401-A41

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human, mouse, Xenopus

Clonality

Polyclonal

Application

ELISA : 1:15,000 - 1:60,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.2 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

SMAD4

SMAD4 Antibody Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. SMAD4 (also known as Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, deletion target in pancreatic carcinoma 4 and hSMAD4) is a common mediator of signal transduction by TGF-b (transforming growth factor), but is also involved in cancer development and metastases as a tumor suppressor. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may form trimers with receptor-regulated SMAD (R-SMAD) and interacts with ATF2, COPS5, DACH1, MSG1, SKI and TRIM33. In the absence of ligand SMAD4 is found in the cytoplasm, but when complexed with R-SMAD, translocates to the nucleus. Defects in SMAD4 are a cause of pancreatic carcinoma and juvenile polyposis syndrome (JPS), a syndrome in which patients are at risk for developing gastrointestinal cancers.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human SMAD4 protein.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to SMAD4 protein by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This affinity purified antibody is directed against human SMAD4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with SMAD4 protein from human, mouse and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Karlsson,G., Blank,U., Moody,J.L., Ehinger,M., Singbrant,S., Deng,C.X. and Karlsson,S. (2007) Smad4 is critical for self-renewal of hematopoietic stem cells. J. Exp. Med. 204 (3), 467-474. Wang,L.H., Kim,S.H., Lee,J.H., Choi,Y.L., Kim,Y.C., Park,T.S., Hong,Y.C., Wu,C.F. and Shin,Y.K. (2007) Inactivation of SMAD4 tumor suppressor gene during gastric carcinoma progression. Clin. Cancer Res. 13 (1), 102-110. Lim,S.K. and Hoffmann,F.M. (2006) Smad4 cooperates with lymphoid enhancer-binding factor 1/T cell-specific factor to increase c-myc expression in the absence of TGF-beta signaling. Proc. Natl. Acad. Sci. U.S.A. 103 (49), 18580-18585. Liang,M., Melchior,F., Feng,X.H. and Lin,X. (2004) Regulation of Smad4 sumoylation and transforming growth factor-beta signaling by protein inhibitor of activated STAT1. J. Biol. Chem. 279 (22), 22857-22865. Roelen,B.A., Cohen,O.S., Raychowdhury,M.K., Chadee,D.N., Zhang,Y., Kyriakis,J.M., Alessandrini,A.A. and Lin,H.Y. (2003) Phosphorylation of threonine 276 in Smad4 is involved in transforming growth factor-beta-induced nuclear accumulation. Am. J. Physiol., Cell Physiol. 285 (4), C823-C830.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra