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THRA Antibody

Rabbit Polyclonal
NCI Collaboration
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Western blot using Rockland's affinity purified anti-THRA antibody shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3).  No staining is evident in lysates from mock-transfected 293 cells (lane 2).  Endogenous THRA is not detected in mouse brain whole cell lysate (lane 4).   Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus.  The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA.  Personal communication, S. Cheng and H. Ying, NCI, Bethesda, MD.
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Number:
600-401-A38
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

THRA Antibody Properties

Description
Anti-Thyroid Hormone Receptor alpha (THRA) (RABBIT) Antibody - 600-401-A38
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:300,000
IF Microscopy : 1:200
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 1:200
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
0.89 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
THRA
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THRA Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Thyroid hormone receptor alpha is a nuclear hormone receptor with high affinity for the hormone triiodo-thyronine.  THRA is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having a certain extent of redundancy, may mediate different functions of thyroid hormone.  THRA interacts with NCOA3 and NCOA6 co-activators, leading to a strong increase in transcription of target genes. THRA is localized within the nucleus and has been found to exist as 4 isoforms originating from alternative splicing variants.  This antibody recognizes THRA isoform 1.  Isoform 1 has a distinct C-terminus compared to isoform 2.
Synonyms
Thyroid hormone receptor alpha Nuclear receptor subfamily 1 group A member 1 c-erbA-alpha c-erbA-1 V-erbA-related protein 7 EAR-7 TRa1
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human THRA isoform 1 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human THRA protein.  A BLAST analysis was used to suggest cross-reactivity with THRA from mouse, human and rat based on a 100% homology with the immunizing sequence.  Cross-reactivity with THRA from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Ying,H., Araki,O., Furuya,F., Kato,Y. and Cheng,S.Y. (2007) Impaired adipogenesis caused by a mutated thyroid hormone alpha1 receptor.  Mol. Cell. Biol. 27 (6), 2359-2371. Nascimento,A.S., Dias,S.M., Nunes,F.M., Aparicio,R., Ambrosio,A.L., Bleicher,L., Figueira,A.C., Santos,M.A., de Oliveira Neto,M., Fischer,H., Togashi,M., Craievich,A.F., Garratt,R.C., Baxter,J.D., Webb,P. and Polikarpov,I.  (2006) Structural rearrangements in the thyroid hormone receptor hinge domain and their putative role in the receptor function. J. Mol. Biol. 360 (3), 586-598. Wan,W., Farboud,B. and Privalsky,M.L. (2005) Pituitary resistance to thyroid hormone syndrome is associated with T3 receptor mutants that selectively impair beta2 isoform function.  Mol. Endocrinol. 19 (6), 1529-1542.
Specific Reference
López-Juárez, A., Remaud, S., Hassani, Z., Jolivet, P., Pierre Simons, J., Sontag, T., ... & Demeneix, B. A. (2012). Thyroid Hormone Signaling Acts as a Neurogenic Switch by Repressing Sox2 in the Adult Neural Stem Cell Niche. Cell stem cell, 10(5), 531-543.
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-218

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
600-401-281

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, IP
Reacts With
Human
Catalog Number
600-401-A38S

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, mouse, rat
Catalog Number
600-401-A96

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry