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Selenoprotein W Antibody

Rabbit Polyclonal
NCI Collaboration
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Number:
600-401-A29S
Size:
25 µL
Price:
$95.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Selenoprotein W Antibody Properties

Description
Anti-Selenoprotein W (RABBIT) Antibody - 600-401-A29S
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse
Clonality
Polyclonal
Application
ELISA : 1:2,500 - 1:10,000
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 1:100 - 1:1,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.38 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SEPW1
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Selenoprotein W Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Selenoprotein W was first purified and characterized from rat skeletal muscle.  The function of selenoprotein W is not entirely clear, but the presence of the bound glutathione moiety indicates that SelW is thought to function in oxidation–reduction catalysis and may play a role in selenium deficiency disorders such as white muscle disease in sheep and Keshan disease in humans. Recently, overexpression of SelW was shown to be glutathione dependent and was shown to markedly reduce the sensitivity of cell lines to H2O2 cytotoxicity.
Synonyms
Selenoprotein W SelW
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full-length mouse SelW protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 9.6 kDa in size corresponding to SelW by western blotting in the appropriate cell lysate or extract.  This antibody is capable of detecting both overexpressed and endogenous SelW.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for mouse Selenoprotein W.  A BLAST analysis was used to suggest cross-reactivity with SelW from rat based on a 98% homology with the immunizing sequence.  Partial reactivity is expected against human-derived SelW based on an 87% homology with the immunogen.  Cross-reactivity with SelW from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Whanger, P.D. (2000) Selenoprotein W: a review. Cell Mol. Life Sci. 57(13-14):1846-52. Whanger, P.D., Vendeland, S.C., Gu, Q.P., Beilstein, M.A. and Ream, L.W. (1997) Selenoprotein W cDNAs from five species of animals. Biomed. Environ. Sci. 10(2-3):190-7. Yeh, J.Y., Beilstein, M.A., Andrews, J.S. and Whanger, P.D. (1995) Tissue distribution and influence of selenium status on levels of selenoprotein W. FASEB J. 9(5):392-6. Gu, Q.P., Sun, Y., Ream, L.W. and Whanger, P.D. (2000) Selenoprotein W accumulates primarily in primate skeletal muscle, heart, brain and tongue. Mol. Cell Biochem. 204(1-2):49-56. Vendeland, S.C., Beilstein, M.A., Yeh, J.Y., Ream, W. and Whanger, P.D. (1995) Rat skeletal muscle selenoprotein W: cDNA clone and mRNA modulation by dietary selenium.  Proc. Natl Acad. Sci. USA 92(19):8749-53.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
bovine, human, mouse, rat, sheep
Catalog Number
100-4151

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
bovine
Catalog Number
100-4191

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
mouse
Catalog Number
200-401-999

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry