You may also want to consider these related products.

Anti-CATALASE (RABBIT) Antibody - 100-4151

View Product.

Anti-SUPEROXIDE DISMUTASE (RABBIT) Antibody - 100-4191

View Product.

Anti-Selenophosphate Synthetase 2 (SPS2) (RABBIT) Antibody - 200-401-999

View Product.

Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302

View Product.

Primary Antibodies > Sample Size Primary Antibodies

Selenoprotein W Antibody

Rabbit Polyclonal

Code

600-401-A29S

Size

25 µL

Price

$89.00

Availability

Ships next business day.

Order Offline:

Phone: 800.656.7625

E-mail: orders@rockland-inc.com

Fax: 610.367.7825

Bulk Orders:

Call or E-mail Us For Information
Fill out our Contact Form

Shipping & Handling Fees Apply.

Shipping:

$85.00 to United States

Handling:

$45.00

Synonyms: Selenoprotein W SelW

Datasheet
References
Customer Reviews
Protocols
Related Products

Selenoprotein W Antibody Properties

Description

Anti-Selenoprotein W (RABBIT) Antibody - 600-401-A29S

Host

Rabbit

Target Species

Mouse

Known Cross Reactivity

mouse

Clonality

Polyclonal

Application

ELISA : 1:2,500 - 1:10,000
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 1:100 - 1:1,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.38 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

SEPW1

Selenoprotein W Antibody Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Selenoprotein W was first purified and characterized from rat skeletal muscle. The function of selenoprotein W is not entirely clear, but the presence of the bound glutathione moiety indicates that SelW is thought to function in oxidation–reduction catalysis and may play a role in selenium deficiency disorders such as white muscle disease in sheep and Keshan disease in humans. Recently, overexpression of SelW was shown to be glutathione dependent and was shown to markedly reduce the sensitivity of cell lines to H₂O₂ cytotoxicity.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full-length mouse SelW protein.

Immunogen Type

Recombinant Protein

Storage Condition

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 9.6 kDa in size corresponding to SelW by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both overexpressed and endogenous SelW.

Purity/Specifity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for mouse Selenoprotein W. A BLAST analysis was used to suggest cross-reactivity with SelW from rat based on a 98% homology with the immunizing sequence. Partial reactivity is expected against human-derived SelW based on an 87% homology with the immunogen. Cross-reactivity with SelW from other sources has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Whanger, P.D. (2000) Selenoprotein W: a review. Cell Mol. Life Sci. 57(13-14):1846-52. Whanger, P.D., Vendeland, S.C., Gu, Q.P., Beilstein, M.A. and Ream, L.W. (1997) Selenoprotein W cDNAs from five species of animals. Biomed. Environ. Sci. 10(2-3):190-7. Yeh, J.Y., Beilstein, M.A., Andrews, J.S. and Whanger, P.D. (1995) Tissue distribution and influence of selenium status on levels of selenoprotein W. FASEB J. 9(5):392-6. Gu, Q.P., Sun, Y., Ream, L.W. and Whanger, P.D. (2000) Selenoprotein W accumulates primarily in primate skeletal muscle, heart, brain and tongue. Mol. Cell Biochem. 204(1-2):49-56. Vendeland, S.C., Beilstein, M.A., Yeh, J.Y., Ream, W. and Whanger, P.D. (1995) Rat skeletal muscle selenoprotein W: cDNA clone and mRNA modulation by dietary selenium. Proc. Natl Acad. Sci. USA 92(19):8749-53.

Specific Reference

There are no reviews for this product. Log in to submit your own review.

AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra