14-10_house_marker_thin
 
You may also want to consider these related products.
Anti-Human p300 Transcription Factor (MOUSE) Monoclonal Antibody - 100-301-176
Anti-Human p300 Transcription Factor (MOUSE) Monoclonal Antibody - 100-301-176
Anti-c-myc (RABBIT) Antibody - 100-401-224
Anti-c-myc (RABBIT) Antibody - 100-401-224
Anti-p53 (MOUSE) Monoclonal Antibody - 200-301-174
Anti-p53 (MOUSE) Monoclonal Antibody - 200-301-174
Anti-AKT pS473 (MOUSE) Monoclonal Antibody - 200-301-268
Anti-AKT pS473 (MOUSE) Monoclonal Antibody - 200-301-268
Primary Antibodies  >  Phospho Specific Antibodies
 

PUMA Antibody

Rabbit Polyclonal
Code

600-401-987
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Immunocytochemistry of PUMA in K562 cells with PUMA antibody at 1 µg/ml.
Western blot analysis of PUMA expression in human (H) K562 and mouse (M) 3T3 cell lysates with PUMA antibody at 2 µg/ml.
Shipping & Handling Fees Apply.

Shipping:
$20.00 to United States
Handling:
$20.00
Synonyms: BCL2 binding component 3 antibody, JFY 1 antibody, JFY1 antibody, p53 up regulated modulator of apoptosis antibody

PUMA Antibody Properties

Description
Anti-PUMA (p53 Upregulated Modulator of Apoptosis) (RABBIT) Antibody - 600-401-987
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse
Clonality
Polyclonal
Application
ELISA : User Optimized
Western Blot : 1-2 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
BBC3
Download CofA
Lot#  

PUMA Antibody Description

Background
Apoptosis is related to development as well as to many diseases.  The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes.  A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3).  PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members.  This family of proteins also includes Bax and Noxa, which are also transcriptional targets of p53.  The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-ß (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53.  PUMA may be a direct mediator of p53-induced apoptosis. Anti-PUMA antibody is ideal for investigators involved in Apoptosis reseach.
Immunogen
PUMA Antibody was produced from whole rabbit serum prepared by repeated immunizations with asynthetic peptide corresponding to amino acids near the c-terminus of human PUMA-a. This sequence is identical between a and b forms of the PUMA proteins.
Immunogen Type
Peptide
Storage Condition
Store vial at 2° - 8° C or below prior to opening. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening product.
Application Note
Anti-PUMA antibody has been tested for use in ELISA, ICC, IF, western blotting and immunocytochemistry.  K562 or 3T3 cell lysates can be used as positive controls in western blotting, and a band at approximately 23 kDa can be detected.  A lower band at approximately 16 kDa was detected in MOLT4 and U937 cell lysates, which may represent the PUMA-b form. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-PUMA Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with PUMA with Human and Mouse based on 100% homology with the immunizing sequence. Cross-reactivity with PUMA from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
1. Nakano K, Vousden KH (2001) PUMA, a novel proapoptotic gene, is induced by p53. Mol. Cell 7:683-694. 2. Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B (2001) PUMA induces the rapid apoptosis of colorectal cancer cells. Mol. Cell 7:673-682. 3. Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T (2001) Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc. Natl. Acad. Sci. USA 98:11318-11323.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Human
Catalog Number
100-301-176

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-224

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP, ChIP
Reacts With
human
Catalog Number
200-301-174

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry