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Primary Antibodies  >  Cancer Research Antibodies
 

ABCB6 Antibody

Rabbit Polyclonal
Code

600-401-945
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western blot using Rockland's affinity purified anti-ABCB6 antibody (right panel, lane B6) shows detection of Flag tagged human ABCB6 protein at 104 kDa and a truncated form of the protein at 79 kDa (arrowheads).  The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein.  A lysate prepared from KB cells without a vector insert (CV lane) should no reactivity with the antibody.  The left panel shows a similar staining pattern using an anti-Flag™ antibody as a positive control.  The membrane was probed with the primary antibody diluted to 1:1,400. Personal Communication, Jill Paterson,  CCR-NCI, Bethesda, MD.
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Synonyms: ABC antibody, ABC transporter umat antibody, ABC14 antibody, ATP binding cassette sub family B member 6, mitochondrial precursor antibody

ABCB6 Antibody Properties

Description
Anti-ABCB6 (RABBIT) Antibody - 600-401-945
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, dog
Clonality
Polyclonal
Application
ELISA : 1:40,000 - 1:130,000
Western Blot : 1:1,000 - 1:5,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
ABCB6
Download CofA
Lot#  

ABCB6 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. ABCB6 (ATP-binding cassette sub-family B member 6, also known as mitochondrial precursor ABC transporter 3, Mt-ABC transporter 3, and ABC transporter umat) is a member of the superfamily of ATP-binding cassette (ABC) transporters.  ABCB6 is a membrane-associated protein that transports various molecules across the extra- and intra-cellular membranes.  ABC genes are divided into seven distinct subfamilies (ABC1, DR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily confer multi-drug resistance (MDR) to cancer cells and are also involved in antigen presentation. ABCB6 also likely plays a role in mitochondrial function. Localized to 2q26, this gene is considered a candidate gene for lethal neonatal metabolic syndrome, a disorder of mitochondrial function.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 440-455 of human ABCB6 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 94 kDa in size corresponding to ABCB6 protein by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity-purified antibody is directed against human ABCB6 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross reactivity with ABCB6 protein from human and dog based on 100% homology with the immunizing sequence.  Expect partial reactivity with ABCB6 from mouse and rat sources based on partial (~93%, 15/16) sequence homology.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Mitsuhashi,N., Miki,T., Senbongi,H., Yokoi,N., Yano,H., Miyazaki,M., Nakajima,N., Iwanaga,T., Yokoyama,Y., Shibata,T. and Seino,S. (2000) MTABC3, a novel mitochondrial ATP-binding cassette protein involved in iron homeostasis. J. Biol. Chem. 275 (23), 17536-17540.
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry