14-10_house_marker_thin
 
You may also want to consider these related products.
Anti-EGFR (RABBIT) Antibody - 100-401-149
Anti-EGFR (RABBIT) Antibody - 100-401-149
Anti-VEGF-165 (RABBIT) Antibody - 209-401-B99
Anti-VEGF-165 (RABBIT) Antibody - 209-401-B99
Anti-EGFR (RABBIT) Antibody - 600-401-905
Anti-EGFR (RABBIT) Antibody - 600-401-905
Anti-EGFR pY1197 (RABBIT) Antibody - 600-401-928
Anti-EGFR pY1197 (RABBIT) Antibody - 600-401-928
Primary Antibodies  >  Kinase and Phosphatase Antibodies
 

p44 Map Kinase Antibody

Rabbit Polyclonal
Code

600-401-943
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western blot using Rockland's Affinity Purified anti-p44  MAP Kinase (ERK1) antibody shows detection of ERK1 in whole cell lysates prepared from mouse brain (left), mouse kidney (center) and mouse intestine (right).
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: ERK1 antibody, ERT 2 antibody, ERT2 antibody, Extracellular Signal Regulated Kinase 1 antibody, Extracellular signal related kinase 1 antibody, HGNC6877 antibody, HS44KDAP antibody, HUMKER1A antibody

p44 Map Kinase Antibody Properties

Description
Anti-p44 MAP Kinase (ERK1) [RABBIT) Antibody - 600-401-943
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:2,000 to 1:10,000
Western Blot : 1:1,000-1:,2000
ImmunoPrecipitation: 5.0 µg/mg sample
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
MAPK3
Download CofA
Lot#  

p44 Map Kinase Antibody Description

Background
Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK’s) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively.  When activated, ERK’s form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the up-regulation of immediate early genes.  As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli.  Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p44 MAP Kinase (ERK1) protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, immunoprecipitation and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 44 kDa in size corresponding to p44 MAP Kinase (ERK1) by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity-purified antibody is directed against the human p44 MAP Kinase (ERK1) protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross reactivity with p44 MAP Kinase (ERK1) proteins from human, bovine, rabbit, rat and mouse. No reactivity is observed against p42 MAP Kinase (ERK2). Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, mouse, rat
Catalog Number
100-401-149

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-B99

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
600-401-905

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-928

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry