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Primary Antibodies  >  Enzyme Antibodies
 

Myosin Antibody

Rabbit Polyclonal
Code

600-401-938
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's anti- RLC of Smooth and Non-muscle Myosin antibody to detect vascular myosin (rat aorta, lane 1) but not cardiac myosin (mouse heart, lane2).  Each lane was loaded with 35 µg of lysate.   Arrowheads indicate the detection of both mono-phosphorylated (upper) and unphosphorylated (lower) forms of the protein.   After blocking with 5% NGS and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted in blocking buffer to 1:600 for 2 h at room temperature.  The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature (800 nm channel, green).   Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).  IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.
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Synonyms: Myosin regulatory light polypeptide 9 Myosin regulatory light chain 9 Myosin regulatory light chain MRLC1 Myosin regulatory light chain 2, smooth muscle isoform Myosin RLC MLC-2C 20 kDa myosin light chain

Myosin Antibody Properties

Description
Anti-Myosin (RABBIT) Antibody - 600-401-938
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:500 - 1:2,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.71 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
MY19
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Lot#  

Myosin Antibody Description

Background
Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail.  The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons.  Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction.   Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain.  The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.  This sequence differs from that of the sarcomeric/
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 12-27 of human myosin light chain protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity-purified antibody was tested by ELISA and immunoblotting and was found to be reactive with both the unphosphorylated and mono-phosphorylated forms of the protein.   Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Purity/Specifity
This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Cross reactivity is expected with myosin light chain from human, mouse and rat sources.  Reactivity with the protein from other species has not been determined; however, the sequence of the immunogen is nearly identical in mammalian and avian species.  BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences.  Cross reactivity is expected.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Kumar,C.C., Mohan,S.R., Zavodny,P.J., Narula,S.K. and Leibowitz,P.J. (1989) Characterization and differential expression of human vascular smooth muscle myosin light chain 2 isoform in nonmuscle cells. Biochemistry 28 (9), 4027-4035. Iwasaki,T., Murata-Hori,M., Ishitobi,S. and Hosoya,H. (2001) Diphosphorylated MRLC is required for organization of stress fibers in interphase cells and the contractile ring in dividing cells. Cell Struct. Funct. 26 (6), 677-683.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human
Catalog Number
600-401-416

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
Mouse, Rat, Human, Monkey, Sheep, Bovine, Goat, Guinea Pig, Swine, Hamster
Catalog Number
600-401-886

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, mouse, chimpanzee, rat
Catalog Number
600-401-959

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry